人参皂苷酶Ⅲ型转化制备人参稀有皂苷Gyp17和Gyp75  

Preparation of rare ginsenoside Gyp17 and Gyp75 by ginsenosidase type Ⅲ

作　　者：庄子瑜 刘春莹 李秋宏[2] 李鹏飞 徐龙权[2] 鱼红闪[2] ZHUANG Ziyu;LIU Chunying;LI Qiuhong;LI Pengfei;XU Longquan;YU hongshan(School of Life Science and Biotechnology,Dalian University,Dalian 116622,China;College of Biotechnology,Dalian Polytechnic University,Dalian 116034,China;Dalian Center for Certification and Food and Drug Control,Dalian 116021,China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]大连工业大学生物工程学院,辽宁大连116034 [3]大连市检验检测认证技术服务中心,辽宁大连116021

出　　处：《食品与发酵工业》2020年第21期42-47,共6页Food and Fermentation Industries

基　　金：国家高端外国专家项目(GDT20152100019)。

摘　　要：为了高效制备稀有人参皂苷Gyp17和Gyp75,利用大肠杆菌克隆表达人参皂苷酶Ⅲ型酶,转化低活性人参皂苷Rb1,制备稀有人参皂苷Gyp17和Gyp75。结果表明,将20 g/L的Rb1皂苷在37℃酶反应24 h,4 g Rb1底物制备了3.1 g稀有皂苷Gyp17,其摩尔得率为90.8%。5 g/L的皂苷Rb1在37℃酶反应60 h,4 g Rb1底物制备了2.1 g Gyp75单体和0.45 g Gyp17单体,其摩尔得率分别为74.2%和13.1%。经HPLC检测,所得到Gyp75和Gyp17皂苷单体纯度均在90%以上。该研究为人参稀有皂苷Gyp17和Gyp75的产业化提供依据。For efficient preparation of rare ginsenoside Gyp17 and Gyp75 from ginsenoside Rb1,a ginsenosidase type Ⅲ was cloned and expressed in Escherichia coli.The results showed that when20 g/L ginsenoside Rb1 was enzymatically catalyzed at 37 ℃ for 24 h,3.1 g rare ginsenoside Gyp17 monomer was obtained from 4 g Rb1;with a molar yield of 90.8%.When 5 g/L ginsenoside Rb1 was enzymatically catalyzed at 37 ℃ for 60 h,2.1 g Gyp75 and 0.45 g Gyp17 monomers were obtained from 4 g Rb1;with a molar yields of 74.2% for Gyp75,and 13.1% for Gyp17.The purities of resultant Gyp17 and Gyp75 were both over 90% according to HPLC analysis.The results provide references for industrial production of Gyp75 and Gyp17.

关 键 词：人参皂苷酶Ⅲ型 生物转化 人参皂苷Gyp17 人参皂苷Gyp75 人参皂苷RB1 

分 类 号：TQ28[化学工程—有机化工]