DEHP胁迫下萝卜的亚显微结构及应答转录组分析  

Submicroscopic structure and transcriptome analysis of radish in DEHP stress

作　　者：冉咏兰 阚建全[1] RAN Yonglan;KAN Jianquan(College of Food Science,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,重庆400715

出　　处：《食品与发酵工业》2020年第21期98-106,共9页Food and Fermentation Industries

摘　　要：为探究邻苯二甲酸二辛酯(diethylhexyl phthalate,DEHP)胁迫下蔬菜差异基因的表达调控,以萝卜为试验材料,以空白处理为对照,在不同DEHP浓度(10、30、50 mg/kg)处理下,利用透射电镜观察萝卜块根亚显微结构,运用转录组高通量测序技术(RNA sequencing,RNA-Seq)技术对萝卜块根样品进行应答转录组分析。结果表明:DEHP胁迫会破坏萝卜块根细胞的结构,导致细胞壁加厚变形,部分断裂,线粒体嵴数减少,内部嵴断裂变模糊甚至解体,且污染浓度越大,破坏越严重。在应答转录组分析中,10、30和50 mg/kg 3个污染组分别获得52707576、47261670和48651920条Clean Reads,与对照组相比,分别产生9885、9385和11880个差异表达基因。基因本体(Gene Ontology,GO)分析结果显示:差异表达基因主要富集在代谢过程、刺激响应和催化活性等生物学过程中。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析结果表明:差异表达基因主要参与苯丙氨酸代谢、亚油酸代谢、α-亚麻酸代谢、苯丙氨酸、酪氨酸和色氨酸的生物合成、甘氨酸、丝氨酸和苏氨酸代谢、苯丙酸生物合成、类胡萝卜素生物合成、半胱氨酸与蛋氨酸代谢等过程,其中丙氨酸-乙醛酸转氨酶、精胺合成酶、脂氧合酶和乙酰辅酶A酰基转移酶等通过半胱氨酸与蛋氨酸代谢、亚油酸代谢和α-亚麻酸代谢参与了萝卜的应答胁迫反应。以上数据将有助于揭示萝卜块根在DEHP胁迫下的应答机制和分子基础,以及相关抗性基因的挖掘和分子辅助育种等方面的研究。To explore the expression regulation of differential genes in vegetables under the stress of diethylhexyl phthalate( DEHP),observation of the submicroscopic structure of radish roots was conducted using transmission electron microscope,also,analysis of the response transcriptome by RNASeq technology for radish treated by different concentrations( 10,30 and 50 mg/kg) of DEHP was applied.The results indicated that DEHP stress destroyed the structure of radish root cells,with thickened,deformed or partially fractured cell walls,and with less mitochondrial cristae as well as blurring and even disintegrating internal cristae.Higher concentration of DEHP led to more serious damage.In the analysis of response transcriptome,52 707 576,47 261 670 and 48 651 920 Clean Reads were obtained in three contaminated groups,which generated 9 885,9 385 and 11 880 differentially expressed genes,respectively.Gene Ontology( GO) analysis showed that the differentially expressed genes were mainly concentrated in the biological processes of metabolism,stimulus response and catalytic activity.And Kyoto Encyclopedia of Genes and Genomes( KEGG) analysis indicated that the differentially expressed genes were mainly involved in the metabolism of phenylalanine,linoleic acid,α-linolenic acid,glycine,serine,threonine,cysteine and methionine,biosynthesis of phenylalanine,tyrosine,tryptophan,phenylpropionic acid and carotenoid.Alanine-glyoxylate transaminase,spermine synthase,lipoxygenase,acetyl-CoA C-acetyltransferase participated in the stress response of radish through metabolism of cysteine and methionine,linoleic acid as well as α-linolenic acid.To sum up,these data are helpful for revealing the mechanism and molecular basis of radish roots under DEHP stress,and for mining relevant resistance genes and molecular-assisted breeding.

关 键 词：邻苯二甲酸二辛酯 萝卜 亚显微结构 转录组 差异表达基因 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]