玉米GATA基因家族的全基因组鉴定及热胁迫下的表达分析  

Genome-Wide Identification of Maize GATA Gene Family and Expression Analysis under Heat Stress

作　　者：王延召[1] 周波[1] 韩小花[1] 黄保[1] 鲁晓民[1] 程军玲 王淑凤 聂利红[3] WANG Yanzhao;ZHOU Bo;HAN Xiaohua;HUANG Bao;LU Xiaomin;CHENG Junling;WANG Shufeng;NIE Lihong(Cereal Crop Research,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Yuzhou Agricultural and Rural Bureau,Yuzhou 451670,China;Economic Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院粮食作物研究所,河南郑州450002 [2]禹州市农业农村局,河南禹州451670 [3]河南省农业科学院经济作物研究所,河南郑州450002

出　　处：《河南农业科学》2020年第11期19-25,共7页Journal of Henan Agricultural Sciences

基　　金：河南省农业科学院重大科技成果培育计划项目。

摘　　要：利用生物信息学方法对玉米基因组中的GATA基因家族成员进行鉴定,并对其理化性质、染色体分布、系统进化、基因结构、保守基序进行分析,并分析其在热胁迫前后的表达水平变化,以期为玉米GATA基因功能研究奠定基础。结果表明,在玉米基因组中共鉴定到37个GATA基因,它们不均匀地分布在10条染色体上,均含有CX2CX18CX2C保守基序;进化树分析表明,玉米GATA基因家族可分为A、B、C、D 4组,分别包含21、10、4、2个基因,同一组内的基因具有相似的基因结构。热胁迫处理后,玉米GATA家族成员中表达上调的基因有9个,包含A组5个(Zm00001d010785、Zm00001d023539、Zm00001d041883、Zm00001d025953、Zm00001d031135),B组2个(Zm00001d011771、Zm00001d034751),C组2个(Zm00001d036494、Zm00001d014656);下调的基因有2个,均属于B组(Zm00001d016361和Zm00001d009193)。说明上调表达的这9个GATA基因在应答热胁迫过程中可能发挥重要作用。The members of GATA gene family were identified,their physicochemical characteristics,chromosome distribution,phylogenetic evolution,gene structure and conserved motif were analyzed by bioinformatics,and their expression levels were analyzed under heat stress,so as to provide basis for function study of GATA genes.The results showed that a total of 37 GATA genes were identified,which were unevenly distributed on 10 chromosomes in maize genome,and harbored a conserved motif CX2CX18CX2C.Phylogenetic tree analysis showed that the maize GATA gene family could be divided into four groups,A,B,C and D,containing 21,10,4 and 2 genes,respectively,and the genes in the same group had similar gene structure.Nine GATA genes including five group A genes(Zm00001d010785,Zm00001d023539,Zm00001d041883,Zm00001d025953 and Zm00001d031135),two group B genes(Zm00001d011771 and Zm00001d034751)and two group C genes(Zm00001d036494 and Zm00001d014656)were up-regulated,and two group B GATA genes(Zm00001d016361 and Zm00001d009193)were down-regulated under heat stress.This study suggested the above up-regulated nine GATA genes may play important roles in response to heat stress.

关 键 词：玉米 GATA基因家族 全基因组鉴定 热胁迫 基因表达 

分 类 号：S513[农业科学—作物学]