外源硼对红地球葡萄幼苗生长及抗性相关指标的影响  

Effect of Boron on Growth and Resistance Related Indicators of Red Globe Grape Seedlings

作　　者：杨阳 谭玉超 常睿哲 任凤山 蒋锡龙 吴新颖 YANG Yang;TAN Yuchao;CHANG Ruizhe;REN Fengshan;JIANG Xilong;WU Xinying(Shandong Academy of Grape/Shandong Engineering Research Center for Grape Cultivation and Deep-processing,Ji’nan 250100,China;Yantai Changyu Castel Company,Yantai 264000,China;Shandong Academy of Pesticide Sciences,Ji’nan 250100,China)

机构地区：[1]山东省葡萄研究院/山东省葡萄栽培与精深加工工程技术研究中心,山东济南250100 [2]烟台张裕卡斯特酒庄有限公司,山东烟台264000 [3]山东省农药科学研究院,山东济南250100

出　　处：《河南农业科学》2020年第11期120-127,共8页Journal of Henan Agricultural Sciences

基　　金：山东省人才计划资金支持现代农业产业体系创新团队建设项目(SDAIT-06-21);山东省重点研发计划果园花果精准调控与品质管理技术研究项目(2017CXGC0210)。

摘　　要：为研究外源硼对葡萄生长及抗性相关指标的影响,以1年生红地球葡萄幼苗为试材,设置营养液硼质量浓度为0、0.2、3.1、6.2 mg/L的4个处理,处理121 d后,分析不同质量浓度硼条件下葡萄幼苗的生长量、硼、可溶性糖、淀粉、可溶性蛋白质、脯氨酸、丙二醛含量、SOD(超氧化物歧化酶)、PAX(抗坏血酸过氧化物酶)、POD(过氧化物酶)、CAT(过氧化氢酶)活性等指标。结果表明,不同处理的葡萄幼苗生长出现显著差异,硼质量浓度为3.1 mg/L时有最大新梢生长量和生物质量;葡萄叶片和根系硼含量随外源硼质量浓度增加而增加,高硼(6.2 mg/L)处理的硼含量较高,叶片硼含量为448.0 mg/kg,根系硼含量为75.3 mg/kg;无硼(0 mg/L)处理的新梢生长量、根系可溶性糖含量、叶片可溶性蛋白质含量、叶片脯氨酸含量、叶片SOD活性、叶片CAT活性最小,根系淀粉含量、叶片丙二醛含量和POD活性最高;随着外源硼浓度的增加,根系可溶性糖含量、叶片可溶性蛋白质含量、叶片脯氨酸含量以及叶片CAT活性增加,根系淀粉含量、叶片POD活性降低,叶片SOD和APX活性呈先升高后降低趋势,叶片丙二醛含量呈先降低后升高趋势;高硼(6.2 mg/L)处理有较高的根系可溶性糖含量、叶片可溶性蛋白质含量、叶片脯氨酸含量以及较高的叶片CAT活性。外源硼质量浓度与葡萄叶片和根系硼含量呈显著正相关(r=0.9501,r=0.9894),与根系淀粉含量呈显著负相关(r=-0.9782),与叶片可溶性蛋白质含量呈正相关(r=0.9233),与叶片POD活性呈负相关(r=-0.8496),但不显著。适量的外源硼有利于葡萄幼苗生长,无硼(0 mg/L)和高硼(6.2 mg/L)处理抑制葡萄幼苗生长,并引起抗性相关的生理生化指标的变化。In order to study the effect of boron on the growth and resistance related indicators of grapes,we chosed one-year-old Red Globe grape seedlings as test materials,and treated them by nutrient solution containing 0,0.2,3.1,6.2 mg/L boron respectively.After 121 days of treatment,we analyzed the physiological and biochemical indicators of grape seedlings under different boron concentrations.Those indicators included plant growth,contents of boron,soluble sugar,starch,protein,proline and malondialdehyde,activities of SOD,PAX,POD and CAT and so on.The results showed that there were significant differences in the growth of grape seedlings treated with different concentrations of boron.When the concentration of boron was 3.1 mg/L,the new shoot growth and biomass accumulation were maximum.The boron content of grape leaves and roots increased with the increase of exogenous boron concentration.For the treatment of different boron,the boron contents in leaves and roots were 448.0 mg/kg and 75.3 mg/kg,respectively.The results showed that the shoot growth,root soluble sugar content,leaf soluble protein content,leaf proline content,leaf SOD activity and leaf CAT activity were the lowest,root starch content,leaf MDA content and POD activity were the highest,with the increase of exogenous boron concentration,root soluble sugar content,leaf soluble protein content,proline content,and leaf CAT activity increased,starch content in roots and POD activity in leaves decreased,SOD and APX activities increased first and then decreased,and malondialdehyde content decreased first and then increased.High boron(6.2 mg/L)treatment had higher root soluble sugar content,leaf soluble protein content,leaf proline content and CAT activity.There were significant positive correlations between the concentration of exogenous boron and the concentration of boron in grape leaves and roots(r=0.9501,r=0.9894),significant negative correlation between exogenous boron concentration and starch content in root system(r=-0.9782),positive correlation between ex

关 键 词：红地球葡萄 硼素营养 生长 抗性指标 超氧化物歧化酶 抗坏血酶过氧化物酶 过氧化物酶 过氧化氢酶 

分 类 号：S663.1[农业科学—果树学]