早期胚胎组蛋白H3K9me3与H3K27me3的研究进展  

Research progress on methylation of histones H3K9 and H3K27 in early-stage embryos

作　　者：孙莹莹 马雪山 苏迎春[1] Sun Yingying;Ma Xueshan;Su Yingchun(Reproduction Center of the First Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450052,China)

机构地区：[1]郑州大学第一附属医院生殖医学中心,450052

出　　处：《中华医学遗传学杂志》2020年第11期1296-1300,共5页Chinese Journal of Medical Genetics

摘　　要：随着对早期胚胎发育各阶段的深入探索,尤其是对合子基因组激活以及第一次细胞分化的研究,研究者发现早期胚胎表观遗传学遵循着严密的时空修饰机制。既往研究已确定H3K9me3和H3K27me3对基因组表达的抑制效应,明确它们在基因组中参与了许多重要的生物学事件,如染色质重编程、基因组印记、胚胎干细胞干性维持及体细胞克隆等,但其涉及的详细分子机制和作用机理仍不完全清晰。本文从发育生物学和表观遗传学方面,阐述早期胚胎组蛋白H3K9me3和H3K27me3研究进展,为了解胚胎发育的复杂机制以及进一步完善体外胚胎培养技术提供理论基础。With the in-depth exploration of all stages in early-stage embryos,in particular zygotic genome activation and first cell lineage differentiation,researchers have found that early embryonic epigenetics follows a strict pattern of temporal and spatial modification.Previous studies have determined the inhibitory effect of H3K9me3 and H3K27me3 on genomic expression,and found that they are involved in many core biological events in the genome such as chromatin reprogramming,genomic imprinting,maintenance of embryonic stem cell pluripotency and somatic cell nuclear transfer,though the detailed molecular mechanism has remained elusive.From the point of developmental biology and epigenetics,this article has expounded the research progress on the methylation of H3K9 and H3K27 histones in early-stage embryos,which may provide a clue for the complex mechanism of embryonic development and improvement of culture method for embryos in vitro.

关 键 词：H3K9me3 H3K27me3 表观遗传修饰 基因组印迹 体细胞核移植 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]