丘脑电刺激对脑外伤后昏迷大鼠前额叶皮质区去甲肾上腺素α1受体表达水平的影响  

The effect of stimulation of the central thalamus on the expression ofα1R in the prefrontal cortex after traumatic brain injury

作　　者：江健 熊婧 钟琳阳 方龙君 冯珍[1] Jiang Jian;Xiong Jing;Zhong Linyang;Fang Longjun;Feng Zhen(Department of Rehabilitation Medicine,The First Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第一附属医院康复医学科,南昌330006

出　　处：《中华物理医学与康复杂志》2020年第10期865-869,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金(81660382,81260295)。

摘　　要：目的探讨丘脑电刺激对脑外伤后昏迷大鼠的促醒作用及其可能机制。方法选取健康成年Wistar种系大鼠45只,随机分为空白对照组、假刺激组和刺激组,每组15只,观察其行为学变化,并通过免疫荧光和Western blot技术检测各组大鼠前额叶皮质组织中去甲肾上腺素α1受体(α1R)的含量。结果刺激组中15只大鼠全部出现翻正反射,假刺激组仅8只大鼠出现翻正反射;将各组大鼠前额叶皮质区α1R含量进行比较,发现刺激组α1R含量水平显著高于假刺激组(P<0.05)。结论丘脑电刺激可改善脑外伤后昏迷大鼠的意识状态水平,对促进脑外伤后昏迷大鼠觉醒有积极作用,其机制可能与前额叶皮质去甲肾上腺素α1R表达水平上调有关。Objective To investigate the effect of stimulation of the central thalamus(CT-DBS)on awakening from coma after traumatic brain injury and the mechanisms involved.Methods Forty-five healthy,adult Wistar rats were randomly assigned to either a blank control group,a sham stimulation or a stimulation group,each of 15.CT-DBS was applied in the stimulation group.Behavioral changes were observed,and the content ofα1-adrenergic receptor(α1R)in the prefrontal cortex(PFC)was measured using immunofluorescence and western blotting.Results The varus reflex was found in all 15 rats in the stimulation group,but in only 8 among the sham stimulation group.The averageα1R content in the PFC was significantly higher in the stimulation group than in the sham stimulation group.Conclusions CT-DBS can improve the level of consciousness after traumatic brain injury and promote awakening,at least in rats.The mechanisms may be related to the up-regulation ofα1R expression in the PFC.

关 键 词：丘脑电刺激 脑外伤 昏迷 促醒 去甲肾上腺素α1受体 

分 类 号：R651.15[医药卫生—外科学]