河南省洛阳市并殖吸虫病疫源地流行病学调查  

Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province

作　　者：李云霞[1] 艾琳 邓艳[3] 陈伟奇[3] 朱鑫[1] 杨治国 李克伟[1] 孙毅莹 LI Yun-xia;AI Lin;DENG Yan;CHEN Wei-qi;ZHU Xin;YANG Zhi-guo;LI Ke-wei;SUN Yi-ying(Luoyang Center for Disease Control and Prevention,Luoyang 471023,China;National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research,WHO Collaborating Center of Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai 200025,China;Luoyang Center for Disease Control and Prevention,Zhengzhou 450016,China)

机构地区：[1]洛阳市疾病预防控制中心,洛阳471023 [2]中国疾病预防控制中心寄生虫病预防控制所,国家热带病研究中心,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海200025 [3]河南省疾病预防控制中心,郑州450016

出　　处：《中国寄生虫学与寄生虫病杂志》2020年第5期664-666,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：对河南省洛阳市溪蟹的并殖吸虫感染情况进行调查,确认洛阳市并殖吸虫病的疫源地。于2013年8月至2019年10月陆续在洛阳9县(市)监测点采集并殖吸虫第二中间宿主溪蟹,实验室检测溪蟹并殖吸虫感染情况。囊蚴采用PCR扩增内转录间隔区2 (ITS2)序列、线粒体细胞色素氧化酶1 (CO1)基因进行鉴定。共捕获560只溪蟹,宜阳县、洛宁县、新安县、嵩县和栾川县发现并殖吸虫囊蚴,经形态学和PCR鉴定为斯氏并殖吸虫囊蚴。宜阳县溪蟹感染率和感染度最高,分别为58.33%和11.54个囊蚴/只,新安县、嵩县、洛宁县和栾川县溪蟹的感染率和感染度依次为25.93%和0.33个囊蚴/只、 19.64%和0.23个囊蚴/只、 11.96%和0.12个囊蚴/只、 6.17%和0.06个囊蚴/只。PCR结果显示,宜阳县、新安县、嵩县、洛宁县和栾川县溪蟹分离出的囊蚴样品扩增出的ITS2长度约500 bp,与斯氏并殖吸虫(GenBank登录号为KX129924.1)的相似性为98%~100%。提示宜阳县、洛宁县、新安县、栾川县和嵩县均为并殖吸虫病疫源地,其中宜阳县属于中度流行区,新安县、洛宁县、栾川县、嵩县为轻度流行区。To investigate the status of Paragonimus spp.infection in freshwater crabs in Luoyang City of Henan Province,and identify the epidemic focus of paragonimiasis.Freshwater crabs as the second intermediate host were collected from surveillance sites in 9 counties(cities)of Luoyang City during the time from August 2013 to October 2019.Paragonimus spp.infection in the crabs was detected in laboratory settings.Paragonimus spp.metacercariae were identified by PCR amplification of the sequence of internal transcribed spacer 2(ITS2)and mitochondrial cytochrome oxidase subunit 1(CO1).A total of 560 freshwater crabs were captured,and Paragonimus metacercariae were found in the crabs from Yiyang County,Luoning County,Xin’an County,Song County,and Luanchuan County,and identified as the species P.skrjabini by morphological observation and PCR amplification.The infection rate and infectivity were found highest in the crabs collected from Yiyang County(58.33%,11.54 metacercariae/crab),followed by Xin’an County(25.93%,0.33 metacercariae/crab),Song County(19.64%,0.23 metacercariae/crab),Luoning County(11.96%,0.12 metacercariae/crab),and Luanchuan County(6.17%,0.06 metacercariae/crab).Results of PCR showed that the ITS2 amplification product has a sequence length of 500 bp from the metacercariae isolated from the crabs of Yiyang County,Xin’an County,Songxian County,Luoning County and Luanchuan County,with 98%-100%homology to P.skrjabini(GenBank accession No.KX129924.1).It was recognized that five counties including Yiyang County,Luoning County,Xin’an County,Luanchuan County,and Song County were identified to be the epidemic foci of paragonimiasis among them,Yiyang County was a moderate endemic area,while the others being mild endemic regions.

关 键 词：并殖吸虫 溪蟹 疫源地 调查 洛阳 

分 类 号：R532.22[医药卫生—内科学]