原生质体诱变及高通量筛选选育链霉素高产菌株  

Protoplast mutagenesis and high-throughput screening for high-yield streptomycin-producing strains

作　　者：贾啸静 马静 胡紫薇 赵国忠 Jia Xiao-jing;Ma Jing;Hu Zi-wei;Zhao Guo-zhong(NCPC Huasheng Co.,Ltd.,Hebei Industrial Enterprise Experimental Research Center,Shijiazhuang 052160)

机构地区：[1]华北制药华胜有限公司,河北省工业企业试验研究中心,石家庄052160

出　　处：《中国抗生素杂志》2020年第9期878-882,共5页Chinese Journal of Antibiotics

摘　　要：目的通过诱变和筛选方法的研究,提高灰色链霉菌(Streptomyces griseus)生产链霉素的水平。方法优化灰色链霉菌的原生质体的生成和再生条件,并对得到的原生质体进行紫外诱变,然后利用微孔板高通量方式对获得菌株进行筛选。结果经过诱变选育获得一株菌NP-11703,其链霉素产量在100L罐上比出发菌株提高了21.8%。结论用紫外诱变原生质体,可以有效提高灰色链霉菌产链霉素的能力。结合高通量筛选模型的应用,可大大加快高产菌株的筛选效率。Objective Mutagenesis and screening techniques were used to improve the streptomycin production by Streptomyces griseus.Methods The conditions of protoplast formation and regeneration of Streptomyces griseus were optimized,and the obtained protoplasts were mutagenized by ultraviolet radiation,and then the strains were screened by a high throughput microplate method.Results A strain named NP-11703 was obtained by mutagenesis,and the yield of streptomycin was 21.8%higher than that of the original strain.Conclusion Protoplast mutagenesis by ultraviolet can effectively improve the streptomycin production ability of Streptomyces griseus.Combining with the application of high throughput screening model,the screening efficiency of high-yield strains can be greatly accelerated.

关 键 词：灰色链霉菌 原生质体 诱变 高通量 

分 类 号：R978.1[医药卫生—药品]