芪红胶囊对心衰模型比格犬血流动力学及血氧含量的影响  

Effect of Qihong Capsule on Heart Hemodynamics and Myocardial Consumption of Oxygen in Heart Failure Model Beagle

作　　者：郭浩[1] 任建勋[1] 李磊[1] 刘建勋[1] 王晓峰[2] 任钧国[1] 韩笑[1] 金龙[1] 史跃[1] 马彦雷[1] GUO Hao;REN Jian-xun;LI Lei;LIU Jian-xun;WANG Xiao-feng;REN Jun-guo;HAN Xiao;JIN Long;SHI Yue;MA Yan-lei(Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;Affiliated Hospital of Traditional Chinese Medical,Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]中国中医科学院西苑医院基础医学研究所中药药理北京市重点实验室,北京100091 [2]新疆医科大学附属中医医院,乌鲁木齐830000

出　　处：《中国实验方剂学杂志》2020年第20期67-73,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81530099,81503292)。

摘　　要：目的:从血流动力学及血氧含量等相关指标的角度探讨芪红胶囊对戊巴比妥钠诱导心衰模型比格犬的治疗作用。方法:采用健康成年比格犬30只,随机分为6组,每组6只,分别为正常组,模型组,地高辛片组(40μg·kg^-1),芪红胶囊高、中、低剂量组(2.6,1.3,0.65 g·kg^-1),采用静脉泵注2%戊巴比妥钠的方法建立比格犬心衰模型,以左室内压上升最大速率降低70%作为模型成功的标准,后经十二指肠给予相应药物,用多道生理记录仪分别测量肢体标准Ⅱ导联心电图、冠状动脉血流量、心输出量、左室内压、左室内压上升最大速率。罗氏血氧分析仪测定不同时间点动脉血氧含量、冠状静脉窦血氧含量,并计算心肌氧利用率。结果:比格犬在静脉泵注2%戊巴比妥钠15 min左右,开始出现明显心衰症状,主要表现Ⅱ导心电图PR间期增加,冠脉血流量、左室内压、心输出量、心搏出量、静脉血氧含量显著降低、而心肌氧利用率显著增高,与正常组比较有显著差异(P<0.01),为与模型组比较,1.3 g·kg^-1芪红胶囊在给药后60~120 min显著增加冠脉血流量(P<0.05),其最大增幅为36%,2.6 g·kg^-1芪红胶囊在给药后45~60 min心搏出量增加明显,最大增幅约16%,与模型组比较有显著差异(P<0.05)。同时能够增加冠静脉窦血氧含量,提示心肌供氧量升高,氧用率降低,芪红胶囊1.3 g·kg^-1组给药60 min后能显著增加左室内压上升最大速率(P<0.05),最大增幅约42%。结论:芪红胶囊能够同时增加冠脉血流量及静脉血氧含量,使得心肌养供应充分,氧的利用率降低,在此基础上,芪红胶囊才能进一步增加心输出量和改善心脏功能,从而发挥心衰保护的作用。Objective:To investigate the therapeutic effect of Qihong capsule on pentobarbital sodium induced heart failure in beagle dogs. Method: Thirty healthy adult beagle dogs were randomly divided into 6 groups,6 in each group. They were normal group,model group,digoxin tablet group(40 μg·kg^-1),Qihong capsule high,medium and low dose groups(2.6,1.3,0.65 g·kg^-1). The heart failure model of beagle dogs was established by intravenous infusion of 2% pentobarbital sodium. The success standard of the model was that the maximum rate of rise of left ventricular pressure was reduced by 70%. The corresponding drugs were given through duodenum. The Ⅱ lead electrocardiogram, coronary blood flow, cardiac output, left ventricular pressure and maximum rate of left ventricular pressure rise were measured by multi-channel physiological recorder. The arterial oxygen content and coronary sinus oxygen content were measured by Roche blood oxygen analyzer at different time points,and the myocardial oxygen utilization rate was calculated. Result: After intravenous infusion of 2% pentobarbital sodium for about 15 minutes,beagle dogs began to show obvious symptoms of heart failure. The main manifestations were the increase of PR interval of Ⅱ lead electrocardiogram,the decrease of coronary blood flow,left ventricular pressure,cardiac output,cardiac output,venous oxygen content,and the increase of myocardial oxygen utilization rate(P<0.01)compared with the model group,Qihong capsule significantly increased coronary blood flow at 60^-120 min after treatment(P<0.05). The cardiac output of 2.6 g·kg^-1 Qihong capsule increased significantly at 45-60 min after treatment,with the maximum increase of about 16%,which was significantly different from that of model group(P<0.05). At the same time,it can increase the oxygen content of coronary sinus blood,which indicates that the myocardial oxygen supply is increased and the oxygen utilization rate is decreased. Qihong capsule 1.3 g·kg^-1 group significantly increased the maximum rate of left v

关 键 词：心力衰竭 血流动力学 芪红胶囊 冠脉血流量 血氧含量 

分 类 号：R2-0[医药卫生—中医学] R289