基于STAT3/miRNA反馈环路探讨中医药促脑梗死后血管新生的调控机制  

Regulatory Mechanism of Traditional Chinese Medicine in Promoting Angiogenesis After Cerebral Infarction Based on STAT3/miRNA Feedback Loop

作　　者：杨仁义[1] 周德生[2] 傅馨莹 颜思阳 龚翠兰 马强 刘利娟[2] YANG Ren-yi;ZHOU De-sheng;FU Xin-ying;YAN Si-yang;GONG Cui-lan;MA Qiang;LIU Li-juan(Hunan University of Chinese Medicine,Changsha 410208,China;The First Hospital Affiliated to Hunan University of Chinese Medicine,Changsha 410007,China;Changde Hospital of Hunan University of Chinese Medicine,Changde 415000,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南中医药大学第一附属医院,长沙410007 [3]湖南中医药大学附属常德医院,湖南常德415000

出　　处：《中国实验方剂学杂志》2020年第20期221-228,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81874463);湖南省科技厅科技创新平台与人才计划项目(2017SK4005);湖南省财政中医药项目名院工程院内优势病种项目(rsk-010-013/006-09);湖南省中医药管理局项目(201824,202046,2020037);湖南省教育厅一般项目(18C0404);湖南中医药大学中西医结合一流学科开放基金项目(2019ZXYJH08)。

摘　　要：脑梗死是脑血流供应障碍引起脑组织缺血缺氧,出现相应神经功能症状的临床疾病,是最常见的脑血管病之一,严重威胁到人类健康,脑梗死的防治研究具有重要的社会意义。血管新生是脑梗死内科治疗的关键切入点,信号传导和转录激活子(STAT)/缺氧诱导因子-1(HIF-1)/血管内皮生长因子(VEGF)通路是介导脑梗死后血管新生的重要通路。本文以血管新生为切入点,以STAT/HIF-1/VEGF信号通路的上游分子STAT3,微小RNA(miRNA)为主要研究对象,综述芯片测序、实验研究、中医病机、中医治法的研究成果,结合"转录因子(TF)—miRNA"调控模式与"微观整体"思想,提出在中医基础理论指导下立方的中药复方和有效成分可能通过STAT3/miRNA反馈环路激活STAT/HIF-1/VEGF信号通路促脑梗死后血管新生,为中医药治疗脑梗死疾病提出了深层次的分子机制及新的方向。Cerebral infarction is a clinical disease with corresponding neurological symptoms caused by cerebral ischemia and hypoxia caused by cerebral blood supply disorder. It is one of the most common cerebrovascular diseases and a serious threat to human health. The prevention and treatment of cerebral infarction has an important social significance. Angiogenesis is the key starting point for medical treatment of cerebral infarction,and signal transduction and transcriptional activators(STAT)/hypoxia inducing factor-1(HIF-1)/vascular endothelial growth factor(VEGF)pathway are important pathways to mediate angiogenesis after cerebral infarction. This paper took the angiogenesis as the starting point and the upstream molecules of STAT/HIF-1/VEGF signal pathway STAT3 and miRNA as the main study objects,and comprehensively discussed the results of chip sequencing,experimental research,traditional Chinese medicine(TCM)pathogenesis and TCM treatment. Based on the regulatory mode of "TF-miRNA" and the idea of "micro-whole",it is suggested that under the guidance of the basic theory of TCM,cubic compound prescriptions of TCM and its active components might activate the STAT/HIF-1/VEGF signal pathway through STAT3/miRNA feedback loop to promote angiogenesis after cerebral infarction,which puts forward a deep molecular mechanism and new direction for the treatment of cerebral infarction with TCM.

关 键 词：脑梗死 血管新生 信号传导和转录激活子3(STAT3) 微小RNA(miRNA) 中医药 

分 类 号：R2-0[医药卫生—中医学] R22