应用东乡野生稻回交重组自交系分析水稻耐低氮产量相关性状QTL  

QTL Analysis for Yield Traits Related to Low Nitrogen Tolerance Using Backcrossing Recombinant Inbred Lines Derived from Dongxiang Wild Rice(Oryza rufipogon Griff.)

作　　者：吴婷 李霞[1] 黄得润 黄凤林 肖宇龙[1] 胡标林[1] WU Ting;LI Xia;HUANG Derun;HUANG Fenglin;XIAO Yulong;HU Biaolin(Rice Research Institute,Jiangxi Academy of Agricultural Sciences/Rice National Engineering Laboratory(Nanchang),Nanchang 330200,China;Hunan Rice Research Institute/Key Laboratory of indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley,Ministry of Agriculture and Rural Affairs,Changsha 410125,China;State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement,China National Rice Research Institute,Hangzhou 310006,China)

机构地区：[1]江西省农业科学院水稻研究所/水稻国家工程实验室(南昌),南昌330200 [2]中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室,杭州310006 [3]湖南省水稻研究所农业农村部长江中下游籼稻遗传重点实验室,长沙410125

出　　处：《中国水稻科学》2020年第6期499-511,共13页Chinese Journal of Rice Science

基　　金：江西省自然科学基金资助项目(20171ACB21071);国家自然科学基金资助项目(31760378);农业农村部长江中下游籼稻遗传育种重点实验室开放课题资助项目(2018KLMA02);水稻生物学国家重点实验室开放课题(20200201)。

摘　　要：【目的】东乡野生稻低氮耐性强,是水稻耐低氮育种的重要资源。鉴定东乡野生稻耐低氮基因对研究耐低氮遗传机制、培育耐低氮水稻品种具有重要意义。【方法】利用协青早B//东乡野生稻/协B BC1F12回交重组自交系及其遗传图谱,应用Windows QTL Cartographer 2.5分析施氮肥和不施氮肥下的株高和产量相关性状QTL。【结果】共检测到57个控制株高和产量性状的QTL,分布于10条染色体上的33个区域,单个QTL表型贡献率为3.17%~63.40%,其中32个QTL的增效等位基因来自东乡野生稻。19个QTL在施氮和未施氮条件下均检测到,38个QTL仅在单一环境下检测到显著效应,表明不同施氮水平下水稻性状的遗传机制不同。【结论】43个QTL分别聚集于7条染色体上的14个QTL簇,表明不同性状涉及到共同遗传机制,并可通过分子标记辅助选择方法进行耐低氮有利等位基因的聚合育种。【Objective】Dongxiang wild rice(Oryza rufipogon Griff.)has strong low nitrogen tolerance and is an important germplasm for low nitrogen tolerance improvement.Identification of genes responsible for low nitrogen tolerance in Dongxiang wild rice is of great importance to understand molecular mechanisms of low nitrogen tolerance and develop rice varieties with low nitrogen tolerance.【Method】Quantitative trait loci(QTLs)for plant height and yield traits under low and normal nitrogen conditions was identified using backcrossing recombinant inbred lines(BC1F12)derived from an interspecific cross Xieqingzao B//Dongxiang wild rice/Xieqingzao B and its genetic linkage.【Result】A total of 57 QTLs were detected in 33 regions on all chromosome,except chromosome 4 and 8.They explained individually 3.17%~63.40%phenotypic variation,and 32 QTLs of them had favorable alleles derived from Dongxiang wild rice.Nineteen QTLs were simultaneously detected under both nitrogen treatments,and 38 QTLs were only identified under single nitrogen treatment,suggesting various genetic mechanisms in rice growth and yield formation under low and normal nitrogen conditions.【Conclusion】Fourteen QTL clusters,43 QTLs included,scattered on seven chromosomes,indicating the common genetic-physiological mechanisms behind different traits,and the QTL pyramiding for low nitrogen tolerance can be achieved by molecular marker-assisted selection.

关 键 词：耐低氮 东乡野生稻 产量性状 数量性状基因座 

分 类 号：S511.034[农业科学—作物学]