利用核磁共振技术表征生物大分子的动态特性  

Biomolecular dynamic properties probed by solution NMR

作　　者：牛晓刚 金长文 Xiaogang Niu;Changwen Jin(Beijing Nuclear Magnetic Resonance Center,Beijing 100871,China;College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China;Beijing National Laboratory for Molecular Sciences,Beijing 100871,China)

机构地区：[1]北京核磁共振中心,北京100871 [2]北京大学化学与分子工程学院,北京100871 [3]北京分子科学国家研究中心,北京100871

出　　处：《中国科学：化学》2020年第10期1375-1383,共9页SCIENTIA SINICA Chimica

基　　金：国家重点研发计划(编号:2016YFA0501201)资助项目。

摘　　要：生物大分子的结构是其生物功能的基础.在生命活动中,生物大分子处于不断运动中,同时其构象存在化学交换,因此研究生物大分子的动态特性对于深入理解其结构与功能的关系是十分必要的.液体核磁共振技术可以在原子水平对生物大分子不同位点的动态特性同时进行表征,能够覆盖从皮秒到秒甚至更慢的时间尺度,具有其他研究手段不可比拟的优越性.近年来新技术和新方法的发展使核磁共振技术可以研究更大分子量和更加复杂体系的动态特性,特别是可以研究其他技术方法难以观测到的低丰度激发态.本文介绍了利用核磁共振技术研究生物大分子动态特性的主要方法和近期的一些研究进展.Functions of biomolecules such as proteins and nucleic acids depend on their structures. However, these biomolecules are highly dynamic during biological processes and often interconvert among multiple conformations.Analyses of biomolecular dynamics are essential for a deep understanding of their structure-function relationship.Solution NMR method has unique advantages in analyzing the dynamic properties of biomolecules at the atomic level,while at the same time capturing the dynamic processes ranging from pico-second to second timescales, which is difficult for other biophysical techniques. In particular, recent progress in solution NMR made it more convenient to investigate the sparsely-populated, invisible conformational state. Herein, we summarized some commonly used NMR techniques in the studies of biomolecular dynamics and discuss a few examples of their applications.

关 键 词：核磁共振 蛋白质 动态学 构象交换 

分 类 号：O657.2[理学—分析化学] O629.73[理学—化学]