分而治之——微液滴中的核酸分析化学研究  

Divide and conquer: analytical chemistry of nucleic acids in droplets

作　　者：廖珮宇 黄岩谊[1,2,3,4,5,6] Peiyu Liao;Yanyi Huang(College of Engineering,Peking University,Beijing 100871,China;Beijing Advanced Innovation Center for Genomics,Peking University,Beijing 100871,China;Biomedical Pioneering Center,Peking University,Beijing 100871,China;College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China;Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,China;Institute for Cell Analysis,Shenzhen Bay Laboratory,Shenzhen 518132,China)

机构地区：[1]北京大学工学院,北京100871 [2]北京大学生物医学前沿创新中心,北京100871 [3]北京未来基因诊断高精尖创新中心,北京100871 [4]北京大学化学与分子工程学院,北京100871 [5]北京大学-清华大学生命科学联合中心,北京100871 [6]深圳湾实验室细胞分析研究所,深圳518132

出　　处：《中国科学：化学》2020年第10期1439-1448,共10页SCIENTIA SINICA Chimica

基　　金：国家重点研发计划(编号:2018YFA0108100);国家自然科学基金(编号:21525521,21927802);北京脑科学及类脑研究中心和北京未来基因诊断高精尖创新中心资助项目。

摘　　要：微液滴本身并不是一个全新的概念或者新现象,但随着近年来基于微液滴中的反应来进行生命分析化学研究,并推广至生命科学与医学的应用中,却成为了一个新趋势.微液滴生命分析化学领域的重点研究方向有两个:适合于生物分子高精度定量检测的液滴生成方式;利用均匀微液滴进行超越以往表现的分子检测,特别是与核酸分析结合的应用.本文中,我们结合该领域的发展趋势与本课题组自身的研究经历,梳理了微液滴生命分析化学的关键技术发展和应用,最后从自身研究经历出发提出该领域当今的一些挑战,以期能够引发同行的思考,开拓研究思路.Droplet itself is not an entirely new concept or phenomenon, but as research based on the reaction in droplets accumulates and has been gradually applied in life sciences and biomedicine, it has now become a trend. The most focused topics of bioanalytical chemistry using droplets are(1) droplet generation methods for high-precision quantification of bio-molecules and(2) in-droplet molecular detection with extraordinary performance, especially the applications in nucleic acids analyses. From our experiences in the past years, we tried to sort out some mainstream trends in this field and thus discuss the key technological developments and applications. In the end, we summarized several challenges in this field, wishing these open questions can inspire our peer researchers.

关 键 词：液滴 核酸分析 微流控 微反应器 生化检测 

分 类 号：O652[理学—分析化学]