泥鳅(♀)×大鳞副泥鳅(♂)正交F1代肌肉营养成分分析  

Analysis of Nutritional Components in the Muscle of Misgurnus anguillicaudatus(♀)×Paramisgurnus dabryanus(♂)Orthogonal F1 generation

作　　者：程林慧 吴华东[1] 王自蕊[1] 周颖 孙艺文 吴娜 熊六凤[1] 李福贵 CHENG Lin-hui;WU Hua-dong;WANG Zi-rui;ZHOU Ying;SUN Yi-wen;WU Na;XIONG Liu-feng;LI Fu-gui(College of Animal Science and Technology,Jiangxi agricultural university,Nanchang 330045,China;College of food science and engineering,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学动物科学技术学院,江西南昌330045 [2]江西农业大学食品科学与工程学院,江西南昌330045

出　　处：《江西农业大学学报》2020年第5期960-969,共10页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31860732);江西省现代农业产业技术体系岗位专项(9021107236);江西省教育厅科技计划项目(9232307104,GJJ160366);江西农业大学博士启动基金项目(9232306018)。

摘　　要：【目的】为探究属间杂交对泥鳅肌肉品质的改良效果,对4个F1代泥鳅的肌肉营养成分以及滋味特征进行测定与分析。【方法】以泥鳅自交F1代(M)、大鳞副泥鳅自交F1代(P)、大鳞副泥鳅(♀)×泥鳅(♂)反交F1代(PM)为对照组,对泥鳅(♀)×大鳞副泥鳅(♂)正交F1代(MP)新鲜肌肉中的常规营养成分、氨基酸及核苷酸进行测定与评价分析,【结果】(1)MP粗蛋白质含量分别高于PM、P、M 3个,但差异均不显著(P>0.05);MP的粗脂肪含量低于P、M(P>0.05),显著低于PM(P<0.05);(2)在4个F1代泥鳅的肌肉总氨基酸含量中,雌性与雄性个体存在差异,雌性依次为MF(41.15 mg/g)、MPF(54.95 mg/g)、PMF(55.60 mg/g)、PF(59.89 mg/g),雄性依次为MM(48.44 mg/g)、PMM(53.23 mg/g)、MPM(53.95 mg/g)、PM(58.35 mg/g),表明雌性MP组合肌肉总氨基酸含量高于M低于PM、P,雄性MP组合肌肉总氨基酸含量高于M、PM,低于P;(3)结合氨基酸评分AAS和化学评分CS可以看出,每个组合的蛋氨酸(Met)数值皆为最小值,表明蛋氨酸为4个F1代泥鳅的共同第一限制性氨基酸;(4)呈味核苷酸与游离氨基酸TAV结果显示,仅MPM与MPF的赖氨酸(Lys)的TAV大于1,对呈味具有显著贡献。【结论】综上所述,2个属间杂交F1代(MP与PM)的基本营养成分、总氨基酸含量等均处于2个自交F1代的(M与P)中间,MP营养价值、滋味优于M,接近于PM,劣于P,为泥鳅肌肉品质提升策略及其新品种选育可提供参考依据。[Objective]In order to explore the improvement effect of intergeneric hybridization on muscle quality of loach,the muscle nutritional and edible value of four F1 loach populations were investigated and analyzed in this study.[Method]Taking Misgurnus anguillicaudatus selfing F1 generation(M)and Paramisgurnus dabryanus selfing F1 generation(P),P.dabryanus(♀)×M.anguillicaudatus(♂)inverse cross F1 generation(PM)as the control groups,the levels of routine nutrients,amino acids and nucleotides in fresh muscle of the breeding M.anguillicaudatus(♀)×P.dabryanus(♂)orthogonal F1(MP)were measured and evaluated.[Results]The main results were as follows:(1)the crude protein content of MP was higher than that of PM,P and M group,but the difference was not significant(P>0.05);the crude fat content of MP was lower than those of P and M(P>0.05),while was significantly lower than that of PM(P<0.05)(.2)Obvious differences were detected in the total amino acid content of muscles between male and female loach generated in the present study.The content values of female combinations were orderly presented as MF(41.15 mg/g),MPF(54.95 mg/g),PMF(55.60 mg/g)and PF(59.89 mg/g),and those of male combinations were orderly presented as MM(48.44 mg/g),PMM(53.23 mg/g),MPM(53.95mg/g)and PM(58.35 mg/g),suggesting that the total amino acid content of muscle of MPF combination was higher than that of MF,but lower than those of PMF and PF;the total amino acid content of muscle of MPM combination was higher than those of MM and PMM,but lower than that of PM(.3)According to the amino acid score(AAS)and chemical score(CS),methionine was regarded as the common first restrictive amino acid of the four loach populations.(4)The contents of IMP,Hx and AMP showed that both male and female MP combinations tasted better than M,worse than P and closer to PM.[Conclusion]Thus,the basic nutrients and total amino acid contents of both intergeneric hybridization F1 generations(MP and PM)are in the middle range of those of the two self-crossing F1 generations

关 键 词：泥鳅 大鳞副泥鳅 属间杂交 肌肉 营养成分 氨基酸 核苷酸 

分 类 号：S966.412[农业科学—水产养殖]