星形胶质细胞外显子组中A-to-I RNA编辑位点的高通量测序鉴定  

A-to-I RNA editing in exome of astrocyte analyzed by the high-throughput sequencing

作　　者：王海涛[1] 周海红[1] 闵建平[1] 王涛[1] 王兰[1] 郭欢[1] 张永东[1] 苏海翔[1] Wang Haitao;Zhou Haihong;Min Jianping;Wang Tao;Wang Lan;Guo Huan;Zhang Yongdong;Su Haixiang(Gansu Provincial Academy of Medical Sciences,Lanzhou 730050 China)

机构地区：[1]甘肃省医学科学研究院,甘肃兰州730050

出　　处：《甘肃医药》2020年第8期673-677,共5页Gansu Medical Journal

基　　金：甘肃省卫生行业科研计划项目(项目编号:GSWSKY-2015-34);甘肃省省属科研院所基础条件建设专项(项目编号:18JR2TA014)。

摘　　要：目的:采用高通量测序技术鉴定星形胶质细胞外显子组中A至I RNA编辑。方法:对4个星形胶质细胞样本的转录组和外显子组分别进行高通量测序,利用生物信息学方法进行深度分析,鉴定出星形胶质细胞外显子组中A至I RNA编辑位点。结果:在星形胶质细胞外显子组中共鉴定出1751个A至I RNA编辑位点,其中5’UTR有110个,3’UTR有1390个,编码区有251个,包括118个错义A至I RNA编辑位点。结论:本研究完成了星形胶质细胞外显子组中A至I RNA编辑位点的鉴定,研究结果将有助于深入理解A至I RNA编辑在神经系统疾病发生中的作用机制。Objective:To analyze A-to-I RNA editing in exome of astrocyte by the high-throughput sequencing technique.Methods:Transcriptome and exome of four astrocyte samples were sequenced through high-throughput sequencing technology respectively.Bioinformatic analyses were applied for further study of the sequencing results to identificate A-to-I RNA editing sites in exome of astrocyte.Results:A total of 1751 A-to-I RNA editing sites in exome of astrocyte were identificated,of which 110 sites located on 5’UTRs,1390 sites located on 3’UTRs,251 sites located on CDS,including 118 missense mutations.Conclusion:This study completed identification of A-to-I RNA editing sites in exome of astrocyte.The research findings can contribute to the better understanding of the molecular mechanisms of A-to-I RNA editing in nervous system disease.

关 键 词：星形胶质细胞 外显子组 A-to-I RNA编辑 高通量测序 

分 类 号：R34[医药卫生—基础医学]