强冬性甘蓝型冬油菜抽薹相关基因SVP和SOC1的克隆与表达分析  

Cloning and expression analysis on bolting related genes SVP and SOC1 in strong winterness Brassica napus L.

作　　者：王祺 蒲媛媛 赵玉红 孙柏林 金姣姣 王万鹏 牛早霞 刘丽君 马骊 武军艳 方彦 李学才 孙万仓 WANG Qi;PU Yuan-yuan;ZHAO Yu-hong;SUN Bai-lin;JIN Jiao-jiao;WANG Wan-peng;NIU Zao-xia;LIU Li-jun;MA Li;WU Jun-yan;FANG Yan;LI Xue-cai;SUN Wan-cang(College of Agronomy,Gansu Agricultural University/Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of AridLand Crop Science in Gansu Province/Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学农学院/甘肃省油菜工程技术研究中心/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070

出　　处：《江苏农业学报》2020年第5期1088-1097,共10页Jiangsu Journal of Agricultural Sciences

基　　金：国家现代农业产业技术体系项目(CARS-12);国家“973”计划项目(2015CB150206);科技部项目(2016YFD0101300);甘肃省重大专项(17ZD2NA016-4);甘肃省现代农业产业技术体系项目(GARS-TSZ-1);国家自然科学基金项目(31860388、31960435)。

摘　　要：甘蓝型冬油菜(Brassica napus L.)的冬前抽薹对北方冬油菜生产是极为不利的。SVP(Short vegetative phase)和SOC1(Suppressor of overexpression of constans 1)是与甘蓝型冬油菜抽薹及生长相关的同源基因,为揭示甘蓝型冬油菜抽薹机制,以甘肃农业大学育成的强冬性甘蓝型冬油菜16VHNTS158、16VHNPZ269-1和16VHTS309-10为试验材料,对SVP和SOC1克隆和表达情况进行分析。核酸序列分析结果表明,强冬性甘蓝型冬油菜SVP和SOC1基因的开放阅读框(ORF)长度分别为726 bp和642 bp,分别编码241个和213个氨基酸;2个基因在进化过程中高度保守,属于MADS-box家族成员,编码的蛋白质均为α-螺旋和卷曲环组成的亲水蛋白质,通过对不同抗寒性材料SVP和SOC1氨基酸序列比较,分别发现了4个和9个氨基酸位点突变。荧光定量(qPCR)分析结果表明,SVP基因在未抽薹植株叶中高表达,在抽薹未现蕾及抽薹现蕾植株叶中低表达;SOC1基因相对表达量的变化趋势与SVP基因相反,抽薹现蕾及成熟植株中的SOC1基因相对表达量较未抽薹植株高。由此推测SVP和SOC1基因可能参与甘蓝型冬油菜抽薹及成花的调控,研究结果可为今后选育强冬性甘蓝型冬油菜晚抽薹抗寒品种提供理论依据。The pre-winter bolting of Brassica napus L.is extremely disadvantageous to the production of winter rape in North China.SVP(Short vegetative phase)and SOC1(Suppressor of overexpression of constans 1)are the homologous genes related to bolting and growth in Brassica napus L..In order to reveal the bolting mechanism of winter rape(Brassica napus L.),strong winterness Brassica napus L.strains 16VHNTS158,16VHNPZ269-1 and 16VHTS309-10 bred by Gansu Agricultural University were used as experimental materials to analyse the cloning and expression of SVP and SOC1.The results of nucleotide sequence analysis showed that the open reading frames(ORF)of SVP and SOC1 genes in winter type Brassica napus L.were 726 bp and 642 bp in length,encoding 241 and 213 amino acids respectively.The two genes belonging to MADS-box family are highly conserved in evolution,which encode hydrophilic proteins composed ofα-helix and loop.By comparing the amino acid sequences of SVP and SOC1 in different cold-resistant materials,four and nine amino acid site mutations were found respectively.The results of quantitative real-time PCR(qRT-PCR)showed that SVP gene was highly expressed in the leaves of unbolting plants,but lowly expressed in the leaves of bolting plants and budding plants.The relative expression changes of SOC1 gene were opposite to SVP gene.The relative expression of SOC1 gene in budding plants and mature plants were higher than in unbolting plants.It is suggested that SVP and SOC1 gene may be involved in the regulation of bolting and flower formation in Brassica napus L.,the results of this study can provide theoretical basis for the breeding of cold-resistant and late bolting varieties of strong winterness Brassica napus L.in the future.

关 键 词：甘蓝型冬油菜 抽薹 SVP基因 SOC1基因 基因表达 

分 类 号：S332[农业科学—作物遗传育种] Q786[农业科学—农艺学]