非高龄卵巢功能减退患者血清中抗核抗体、抗心磷脂抗体和抗β2糖蛋白1抗体的检测价值  

Detection of Antinuclear Antibodies,Anticardiolipin Antibodies,and Anti-β2-Glycoprotein 1 Antibodies in Non-Elderly Patients with Diminished Ovarian Reserve

作　　者：张园[1] 夏心如 蒋春艳[1] 舒黎[1] 吴畏[1] 高彦[1] 黄洁[1] 冒韵东[1] 刘嘉茵[1] 马翔[1] ZHANG Yuan;XIA Xin-ru;JIANG Chun-yan;SHU Li;WU Wei;GAO Yan;HUANG Jie;MAO Yun-dong;LIU Jia-yin;MA Xiang(Department of Reproductive Medicine,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院生殖医学科,210029

出　　处：《国际生殖健康／计划生育杂志》2020年第6期445-449,共5页Journal of International Reproductive Health/Family Planning

基　　金：江苏省妇幼保健青年人才项目(FRC201793);东南大学-南京医科大学联合科研攻关项目(JX102GSP2017DN26);江苏省科教强卫工程(YXZXB2016001)。

摘　　要：目的:探讨抗核抗体(ANA)、抗心磷脂抗体(aCL)和抗β2糖蛋白1抗体(aβ2GP1)是否可作为非高龄卵巢功能减退(DOR)患者行体外受精/胞浆内单精子注射(IVF/ICSI)前病因筛查的检测指标。方法:收集2018年1月—2019年4月在南京医科大学第一附属医院生殖医学科接受IVF/ICSI助孕的非高龄DOR患者(非高龄DOR组,127例)和因男方因素行IVF/ICSI助孕的女性患者(对照组,82例)为研究对象,收集血清,采用免疫荧光法检测ANA,酶联免疫吸附法检测aCL和aβ2GP1。结果:非高龄DOR组3项抗体中至少1项阳性的总阳性率(24.41%,31/127)高于对照组(12.20%,10/82),差异有统计学意义(P<0.05);非高龄DOR组ANA阳性率(3.15%,4/127)虽高于对照组(1.22%,1/82),但差异无统计学意义(P>0.05);非高龄DOR组aCL总阳性率(8.66%,11/127)与对照组(6.10%,5/82)比较,差异无统计学意义(P>0.05);非高龄DOR组aβ2GP1阳性率(15.75%,20/127)高于对照组(6.10%,5/82),差异有统计学意义(P<0.05)。结论:非高龄DOR患者aβ2GP1阳性率较高,对非高龄DOR患者推荐在IVF/ICSI助孕前进行血清ANA、aCL和aβ2GP1联合筛查。Objective:To investigate whether antinuclear antibody(ANA),anticardiolipin antibody(aCL)and anti-β2-glycoprotein1 antibody(aβ2GP1)can be used as the screening markers in non-elderly patients with diminished ovarian reserve(DOR)before their IVF/ICSI treatment.Methods:From January 2018 to April 2019,127 non-elderly DOR patients(non-elderly DOR group,127 cases)and 82 women who were planned to undertake IVF/ICSI treatment due to male factors(control group,82 cases)were collected as the subjects.Sera ANA was detected by immunofluorescence,aCL and aβ2GP1 antibodies were detected by enzyme-linked immunosorbent assay.Results:The total positive rate of at least one of the three antibodies in the non-elderly DOR group(24.41%,31/127)was significantly higher than that in the control group(12.20%,10/82),the difference was statistically significant(P<0.05).The total positive rate of ANA in the non-elderly DOR group(3.15%,4/127)was slightly higher than that in the control group(1.22%,1/82),the difference was not statistically significant(P>0.05).The difference of the total positive rate of aCL was not significant between the non-elderly DOR group(8.66%,11/127)and the control group(6.10%,5/82)(P>0.05).The total positive rate of aβ2GP1 in the non-elderly DOR group(15.75%,20/127)was significantly higher than that in the control group(6.10%,5/82),the difference was statistically significant(P<0.05).Conclusions:The combined screening of serum ANA,aCL and aβ2GP1 can be recommended for non-elderly patients with diminished ovarian reserve before IVF/ICSI treatment.

关 键 词：抗体 抗核 抗体 抗心脂 抗Β2糖蛋白1抗体 卵巢功能减退 酶联免疫吸附测定 荧光免疫测定 

分 类 号：R711.75[医药卫生—妇产科学] R446.6[医药卫生—临床医学]