ADSL基因启动子区遗传变异与鸡肉冻藏新鲜度的相关性分析  

Association Analysis Between Genetic Variation in Promoter Region of ADSL Gene and Freshness of Frozen Chicken

作　　者：喻世刚[1] 孙学良 王钢[1] 廖娟[1] 胡强[1] YU Shigang;SUN Xueliang;WANG Gang;LIAO Juan;HU Qiang(Engineering Research Center of Sichuan Province Higher School of Local Chicken Breeds Industrialization in Southern Sichuan,Leshan Normal University,Leshan 614000,China)

机构地区：[1]乐山师范学院,川南地方品种鸡产业化四川省高等学校工程研究中心,乐山614000

出　　处：《中国畜牧兽医》2020年第11期3536-3545,共10页China Animal Husbandry & Veterinary Medicine

基　　金：四川省科技计划资助项目(2018JY0452、2019ZHFP0003);乐山市科技计划项目(18JZD102)。

摘　　要：试验旨在探究鸡腺苷琥珀酸裂解酶(adenylosuccinate lyase,ADSL)基因启动子区多态性及其与鸡肉冻藏新鲜度的相关性。以161只青脚麻鸡为研究对象,通过DNA混池测序法检测ADSL基因启动子区遗传变异。采用等位基因特异性PCR完成个体基因型分析,并分析了各基因型与鸡胸肌肉色、体重及胸肌冷冻60 d后新鲜度K值和pH的相关性。结果显示,在ADSL基因启动子区ATG上游1670 bp处发现1个SNP位点(c.-1670 C>A),其AA基因型个体肌肉冷冻60 d后新鲜度K值(23.61%)极显著低于CA(33.00%)和CC(33.56%)基因型(P<0.01),即冻藏60 d后AA基因型个体肌肉较CA和CC基因型新鲜,CA和CC基因型间新鲜度K值差异不显著(P>0.05);各基因型间鸡体重、胸肌肉色及胸肌冷冻60 d后pH均无显著差异(P>0.05)。生物信息学预测发现,c.-1670 C>A导致ADSL基因启动子区转录因子结合位点的显著改变,该突变位点可能造成ADSL基因的表达差异。综上,ADSL基因启动子区c.-1670 C>A与青脚麻鸡胸肌冷冻60 d后新鲜度K值具有显著相关性,该位点可作为青脚麻鸡肉品新鲜度的候选分子遗传标记。This study was aimed to explore the genetic variations in promoter region of adenylosuccinate lyase(ADSL)and its correlation with chicken freshness after freezing.161 Partridge Shank chickens were employed,the genetic variation of promoter region of ADSL gene was scanned by pool-based sequencing to discover candidate SNP,and the candidate SNP was genotyped using allele specific PCR.The association analysis was performed between the candidate SNP and four indexes(meat color,body weight,freshness(K value)after freezing of 60 days and pH)in both genotypes.The results showed that one SNP(c.-1670 C>A)was identified at 1670 bp upstream of ATG in the promoter region of ADSL gene.The K value of muscle freshness of AA genotype(23.61%)was extremely significantly lower than that of CA(33.00%)and CC(33.56%)genotypes(P<0.01),indicating that the muscle of AA genotype was fresher than that of CA and CC genotypes after freezing for 60 days.No significant difference in meat freshness was observed between CA and CC genotypes(P>0.05).There was no significant difference in the body weight,meat color and pH among three genotypes(P>0.05).Bioinformatics prediction results showed that c.-1670 C>A caused the transcription factor site to change in the promoter region of ADSL gene,which might affect the expression of ADSL gene.This study concluded that there was a significant correlation between c.-1670 C>A in the promoter region of ADSL gene and freshness K value of breast muscle in Partridge Shank chickens after freezing for 60 days,c.-1670 C>A could be used as a candidate molecular genetic marker for meat freshness in Partridge Shank chickens.

关 键 词：青脚麻鸡 ADSL基因 SNP 分子标记 

分 类 号：S831.2[农业科学—畜牧学]