C-型利钠肽调控鸡肌内脂肪细胞脂肪沉积的作用机制研究  

Mechanism of C-type Natriuretic Peptide Regulating Lipid Deposition of Intramuscular Adipocytes in Chickens

作　　者：黄华云[1] 梁忠[1] 刘龙洲[1,2] 赵振华 王钱保[1] 李春苗[1] 黄正洋[1] 张欣[1,3] 黎寿丰 HUANG Huayun;LIANG Zhong;LIU Longzhou;ZHAO Zhenhua;WANG Qianbao;LI Chunmiao;HUANG Zhenyang;ZHANG Xin;LI Shoufeng(Jiangsu Institute of Poultry Science,Yangzhou 225125,China;Yangtze University,Jingzhou 434025,China;Yangzhou University,Yangzhou 225009,China)

机构地区：[1]江苏省家禽科学研究所,扬州225125 [2]长江大学,荆州434025 [3]扬州大学,扬州225009

出　　处：《中国畜牧兽医》2020年第11期3555-3562,共8页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省自然科学基金(BK20191217);江苏省农业科技自主创新资金项目(CX(20)2012);现代农业产业技术体系建设专项(CARS-41-Z05);江苏省成果转化项目(BA2019049);省属公益类科研院所自主科研经费项目(BM2018026-2)。

摘　　要：本研究旨在探讨C型利钠肽(CNP)对鸡胸肌组织肌内前脂肪细胞的增殖、分化和分解的调控作用及机制,为进一步阐明肌内脂肪沉积的分子机制奠定基础。以黄羽肉鸡胸肌组织的肌内前脂肪细胞作为体外研究模型,利用10-7 mol/L CNP激素外源诱导前脂肪细胞,采用CCK8、Edu染色法观察肌内前脂肪细胞增殖的变化,油红O染色和异丙醇萃取法观察脂肪分化和沉积的变化;利用试剂盒检测细胞内cGMP及甘油浓度变化;实时荧光定量PCR检测利钠肽受体A、B、C(NPRA、NPRB和NPRC)的表达变化。结果显示,与对照组相比,CNP诱导前脂肪细胞1和3 d后,细胞的增殖能力显著升高(P<0.05);CNP诱导3和6 d后,诱导组脂肪细胞的分化和脂滴沉积能力极显著降低(P<0.01),释放到培养基的甘油浓度及细胞中的cGMP的浓度极显著升高(P<0.01)。NPRB和NPRC基因mRNA的表达极显著和显著高于对照组(P<0.01;P<0.05),NPRA基因mRNA的表达无显著变化(P>0.05)。本试验结果表明,CNP通过NPRB/NPRC-cGMP通路调控肉鸡肌内脂肪细胞的脂代谢过程。The aim of this study was to investigate the regulatory effect and mechanism of C-type natriuretic peptide(CNP)on the proliferation,differentiation and lipolysis of intramuscular preadipocytes(IMPs)in breast muscle of chickens,and lay a foundation on further elucidating the molecular mechanism of intramuscular fat deposition in chickens.The IMPs of breast muscle in Yellow-feathered broilers were used as the model in vitro,the preadipocytes were induced by 10-7 mol/L CNP.The proliferation,differentiation and lipolysis of IMPs were observed by CCK8,Edu staining,Oil Red O staining and isopropanol extraction,respectively.The concentration of cGMP and glycerin were detected by cGMP and glycerin kit,respectively.The expression of NPRA,NPRB and NPRC genes mRNA was detected by Real-time quantitative PCR.The results showed that compared with control group,the cell proliferation in CNP treatment group was enhanced significantly at 1 and 3 d(P<0.05).The cell differentiation and lipid deposition were extremely significantly decreased at 3 and 6 d(P<0.01),the intracellular cGMP concentration and glycerin concentration in the medium at 3 and 6 d were extremely significantly increased(P<0.01).The expression of NPRB and NPRC genes mRNA in CNP treatment group was extremely significantly or significantly higher than control group(P<0.01;P<0.05),and the expression of NPRA gene mRNA had no significant difference(P>0.05).The results revealed that CNP regulated the lipid deposition of intramuscular adipocytes through NPRB/NPRC-cGMP pathway in chickens.

关 键 词：C-型利钠肽(CNP) 鸡 肌内脂肪细胞 脂肪沉积 

分 类 号：S831.2[农业科学—畜牧学]