表达猪瘟病毒E2蛋白的重组PRRSV疫苗株rPRRSV-E2的水平传播能力研究  

STUDY ON HORIZONTAL TRANSMISSION OF THE RECOMBINANT PRRSV VACCINE STRAIN(RPRRSV-E2)EXPRESSING E2 PROTEIN OF CLASSICAL SWINE FEVER VIRUS

作　　者：高飞[1,2] 姜一峰 李国新[1,2] 张玉娇 虞凌雪[1,2] 周艳君 李丽薇 郑海红[1,2] 童光志 GAO Fei;JIANG Yi-feng;LI Guo-xin;ZHANG Yu-jiao;YU Ling-xue;ZHOU Yan-jun;LI Li-wei;ZHENG Hai-hong;TONG Guang-zhi(l.Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis,Yangzhou University,Yngzhou 225009,China)

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心扬州大学,扬州225009

出　　处：《中国动物传染病学报》2020年第6期19-23,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金项目(31670158,31702240);国家重点研发计划政府间国际科技创新合作重点专项(2016YFE0112500)。

摘　　要：为研究表达猪瘟病毒E2蛋白的重组PRRSV疫苗株rPRRSV-E2能否在猪群中水平传播,本研究将15头PRRSV抗原抗体均为阴性,PRV、CSFV和PCV2抗原阴性的35日龄健康仔猪,随机分成3组,每组5头。A组接种rPRRSV-E2第5代细胞毒,剂量为105.0TCID50/头;B组为同居感染对照组,接种DMEM;Mock组接种DMEM,单独饲养。猪体免疫后第7 d采集血清、鼻拭子和肛拭子样品,进行病毒载量和PRRSV、CSFV的抗原抗体含量测定,共持续监测3个月;所有猪只在试验期间每天观察临床症状。结果显示:未接种疫苗的同居对照组猪只各时间点血清中均未检测到PRRSV,也未检测到针对PRRSV和CSFV的抗体。研究结果表明,rPRRSV-E2不能在猪群中水平传播。In order to investigate whether or not the recombinant PRRSV vaccine strain rPRRSV-E2 expressing the E2 protein of classical swine fever virus(CSFV)had the capability of pig-to-pig transmission,fifteen 35-day-old healthy piglets free of PRRS virus and antibodies,PRV,CSFV and PCV2 were randomly divided into 3 groups with 5 pigs each.Piglets in Group A were vaccinated with a dose of 105.0 TCID50 of rPRRSV-E2 at passage 5.Piglets in Group B were inoculated with DMEM and commingled with Group A piglets.Serum,nasal swab and anal swab samples were weekly collected for 3 months post vaccination and the viral loads were tested by fluorescence quantitative PCR.At the same time,piglets in mock group C was inoculated with DMEM and housed separately.Clinical signs were daily observed for all piglets during the entire experiment.The experimental results showed no PRRSV or antibodies detected in the serum samples of piglets in groups B and C,suggesting that rPRRSV-E2 did not perform horizontal transmission among pigs.

关 键 词：基因工程疫苗 水平传播 中和抗体 病毒分离 

分 类 号：S852.651[农业科学—基础兽医学]