核酸适配体和表面等离子体检测技术用于毒品检验的研究进展  

Recent progress towards the application of aptamer and surface plasmon technique in drug detection

作　　者：王玲 柯浩堃 吕赛男 杜宪超 郝红霞 周红[4] WANG Ling;KE Haokun;LV Sainan;DU Xianchao;HAO Hongxia;Zhou Hong(China University of Political Science and Law,Institute of Evidence Law and Forensic Science,Beijing 100088;State Key Laboratory of Luminescent Materials and Devices,Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates,Center for Aggregation-Induced Emission,South China University of Technology,Beijing 100088;MOE(Ministry of Education)Key Laboratory of Evidence Science,Affiliated under China University of Political Science and Law,Collaborative Innovation Center of Judicial Civilization,Guangzhou 510640;Institute of Forensic Science,Ministry of Public Security,Beijing 100038)

机构地区：[1]中国政法大学证据科学研究院,北京100088 [2]证据科学教育部重点实验室(中国政法大学),2011计划司法文明协同创新中心,北京100088 [3]华南理工大学发光材料与器件国家重点实验室,广东省分子聚集发光重点实验室,聚集诱导发光研究中心,广州510640 [4]公安部物证鉴定中心,北京100038

出　　处：《分析试验室》2020年第10期1233-1240,共8页Chinese Journal of Analysis Laboratory

基　　金：中国政法大学青年教师学术创新团队资助项目(18CXTD09);中国政法大学省部级合作项目(2011-23317015)资助;国家自然科学基金(81871523)。

摘　　要：传统实验室检测技术对毒品具有很好的选择性和灵敏度,但是仍需要开发简单、快速、高效的毒品快速检测手段。核酸适配体具有靶分子广、稳定性好、灵敏度高、特异性强等优点,与表面等离子体技术(SPR)相结合,可以实现无标记、高通量、实时快速监测小分子,被广泛应用于各个领域。然而,基于SPR技术的核酸适配体传感器用于毒品的检测鲜有报道,为此,对核酸适配体和表面等离子共振技术在毒品检测领域的应用特点及发展趋势作了研究,以期为科研人员提供参考和借鉴,从而推动毒品现场快速检测技术的更新与发展。Although traditional laboratory techniques for drug detection have high selectivity and sensitivity,a rapid,simple,fast and effective detection method is still highly desired.Aptamers have the advantages of wide target molecules,good stability,high sensitivity and specificity.Combined with surface plasma technology,they can realize unlabeled,high-throughput and rapid real-time monitoring of small molecules,and were widely used in various fields.However,aptamer biosensor based on surface plasmon resonance for drug detection was rarely reported.In this paper,the application characteristics and development trend of aptamer and surface plasmon resonance in drug detectionare was studied in depth,aiming to provide references for scientists so as to promote the update and development of rapid detection.

关 键 词：毒品 毒品检测 核酸适配体 表面等离子体技术 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]