温州地区水产品中哈维氏弧菌的耐药分析  

Drug Resistance of Vibrio harveyi from Fishery Products in Wenzhou,China

作　　者：吴越[1] 郑伊诺 陆荣茂[1] 王瑶华[1] 闫茂仓[1] 胡园[1] 周朝生[1] WU Yue;ZHENG Yinuo;LU Rongmao;WANG Yaohua;YAN Maocang;HU Yuan;ZHOU Chaosheng(Zhejiang Mariculture Research Institute, Wenzhou 325005, China)

机构地区：[1]浙江省海洋水产养殖研究所,浙江温州325005

出　　处：《水产科学》2020年第6期837-843,共7页Fisheries Science

基　　金：浙江省自然科学基金委员会基础公益研究计划项目(LGN18C190006,LGN19C030001).

摘　　要：为探明温州地区水产品中哈维氏弧菌的耐药性,利用药敏试剂盒快速测定自温州地区海水鱼类、贝类和虾类中分离到的11株经生化鉴定和16S rRNA测序证实的致病性哈维氏弧菌对14种渔药的最小抑菌质量浓度,并通过高通量荧光定量PCR法全面分析菌株中磺胺类、喹诺酮类和四环素类药物抗性基因表达谱。药敏结果显示,11株哈维氏弧菌对磺胺嘧啶和氨苄西林钠的耐药率最高均为100%,对磺胺甲噁唑和磺胺间甲氧嘧啶的耐药率也较高,分别为90.91%和81.82%,而对磺胺二甲氧嘧啶、盐酸环丙沙星、恩诺沙星和强力霉素均敏感。抗性基因检测显示,所有菌株均携带磺胺类、喹诺酮类和四环素类抗性基因,磺胺类抗性基因中sul1检出率最高达81.82%,喹诺酮类中qnrD检出率最高为63.64%,四环素类中tet34、tet35和tet36检出率最高均为100%。试验结果表明,大部分哈维氏弧菌已经产生了对磺胺类和β-内酰胺类药物的多重耐药性,菌株普遍携带多种抗性基因,磺胺类耐药表型和抗性基因的携带存在一定相关性,喹诺酮类和四环素类耐药表型和抗性基因之间则明显不一致。Drug sensitivity analysis kit was used to test the minimum inhibitory concentrations of 14 drugs against 11 strains of Vibrio harveyi identified from the infected fishes,shellfishes and shrimps in maricultyre in Wenzhou,China by biochemical identification and 16S rRNA sequencing,and high-throughput quantitative RT-PCR assay was used to test the expression profile of resistance gane of sulfonamide,quinolone and tetracycline to analyze the drug resistance of V.harveyi from aquatic animals in marine in Wenzhou.Susceptibility test showed that the 11 strains of V.harveyi had the maximal resistance rates(100%)to sulfadiazine and ampicillin sodium,high resistance rates to sulfamethoxazole(as 99.30%)and sulfamethoxine(88.03%),and sensitive to sulfamethazine,ciprofloxacin,enrofloxacin and doxycycline.The three kinds of antibiotic resistance genes were detected positively in all strains,with the maximal detection rate of sul1 gene(81.82%)in all sulfonamide resistance genes,and the maximal detection rate of qnrD gene(63.64%)in all quinolone resistance genes,and the maximal detection rate of tet34,tet35 and tet36(100%)in all tetracycline resistance genes.The findings indicated that most of the strains were multi-drug resistant to sulfonamides andβ-lactam,and universally carried multiple resistance genes.There was a certain relationship between the sulfonamide resistance phenotype and the related resistance genes,while the quinolone,and tetracycline resistance phenotypes were not in direct proportion to the resistance genes.

关 键 词：哈维氏弧菌 耐药性 抗性基因 

分 类 号：S917.1[农业科学—水产科学]