五倍子分离组分抑制无乳链球菌的热动力学研究  

Thermodynamics of Inhibition of Extract from Galla Chinensis on Streptococcus agalactiae

作　　者：郑梦珍 佟延南 翟少伟[1,2,3] 林茂 江兴龙[1,2,3] 赖晓健 李忠琴[1,2,3] ZHENG Mengzhen;TONG Yannan;ZHAI Shaowei;LIN Mao;JIANG Xinglong;LAI Xiaojian;LI Zhongqin(Fisheries College, Jimei University, Xiamen 361021, China;Engineering Research Centre of Eel Modern Industrial Technology, Ministry of Education, Xiamen 361021, China;Fishery Pharmaceutical Engineering and Technological Research Center in Xiamen, Xiamen 361021, China;Hainan Academy of Ocean and Fisheries Sciences, Haikou 570206, China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]鳗鲡现代产业技术教育部工程研究中心,福建厦门361021 [3]厦门市渔用药物工程技术研究中心,福建厦门361021 [4]海南省海洋与渔业科学院,海南海口570206

出　　处：《水产科学》2020年第6期947-952,共6页Fisheries Science

基　　金：现代农业产业技术体系专项资金(CARS-46);“十三五”国家海洋经济创新发展示范项目(16PZY002SF18);福建省海洋生物增养殖与高值化利用重点实验室基金资助项目(2019fjscq03);福建省特种水产配合饲料重点实验室基金资助项目(TMKJZ1906);鳗鲡现代产业技术教育部工程中心开放基金资助项目(RE201903).

摘　　要：为评价五倍子各分离组分对无乳链球菌的抑制作用,以期在实践中更高效地利用五倍子,利用已优化过的乙酸乙酯—乙醇—水(5∶1∶5,体积比)分离溶剂体系,应用高速逆流色谱法分离五倍子各组分。通过微量量热法检测五倍子粗提物和各分离组分对无乳链球菌TN3L的热动力学作用,计算得出五倍子各分离组分对无乳链球菌的半数抑制质量浓度。试验结果表明,五倍子可分离出5个组分(W1、W2、W3、W4、W5);组分W1对无乳链球菌无抑制作用,五倍子粗提物及其他分离组分的半数抑制质量浓度分别为0.1234、2.2294、0.2570、0.6687、0.1750 mg/mL,抑菌强度依次为W2<W4<W3<W5<粗提物,其中组分W5和W3抑菌效果明显,推测W5和W3为五倍子对无乳链球菌的主要抑制成分。The effective components of Galla Chinensis were extracted by using high-speed counter current chromatography(HSCCC)and the optimal separation solvent system consisted of ethyl acetate,ethanol and water(with a volume ratio of 5∶1∶5)and the in vitro antibacterial effects of the crude extract and the separate components on Streptococcus lactis TN3L was detected by microcalorimetry and the half inhibitory concentration of the ingredients of the Galla Chinensis in order to evaluate the inhibitory effect of Galla Chinensis isolates on Streptococcus agalactiae and to utilize gallnut more effectively in practice.The results showed that Galla Chinensis was separated into 5 components including W1,W2,W3,W4,and W5.The IC50 of fractions W2—W5 were found to be 2.2294 mg/mL,0.2570 mg/mL,0.6687 mg/mL,and 0.1750 mg/mL,the descending order of inhibitory effect being described as W2<W4<W3<W5<crude isolate,without inhibitory effect on S.agalactiae TN3L in fraction W1,and strong inhibitory effect in components W5 and W3 which were the main antibacterial fractions of Galla Chinensis on S.agalactiae TN3L.

关 键 词：五倍子 高速逆流色谱法 无乳链球菌TN3L 微量量热法 半数抑制质量浓度 

分 类 号：S948[农业科学—水产养殖]