德国小蠊对高效氯氰菊酯抗性选育与抗性机制研究  

RESISTANCE SELECTION AND MECHANISM OF BLATTELLA GERMANICA TO BETE-CYPERMETHRIN

作　　者：李秋红[1] 刘美德[1] 周小洁[1] 张勇[1] 佟颖[1] 曾晓芃[1] LI Qiu-Hong;LIU Mei-De;ZHOU Xiao-Jie;ZHANG Yong;TONG Ying;ZENG Xiao-Peng(Beijing Center for Disease Prevention and Control,Beijing Center of Preventive Medicine Research,Beijing 100013,China)

机构地区：[1]北京市疾病预防控制中心,北京市预防医学研究中心,北京100013

出　　处：《寄生虫与医学昆虫学报》2020年第3期168-173,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：首都卫生发展科研专项项目(编号:首发2018-2-3015)。

摘　　要：于室内以高效氯氰菊酯对德国小蠊进行抗药性筛选,并探讨其抗药性机理。当选育至10代后抗性倍数增长至近8倍。抗性品系羧酸酯酶(CarE)(比活力13.85 nmol/(min·mg))、谷胱甘肽S-转移酶(GSTs)(比活力7.32)和细胞色素P450(CYP450)(比活力0.15)活性均显著高于敏感品系(CarE 6.85、GSTs 2.71、CYP4500.08)。钠离子通道基因编码序列分析表明,敏感品系均不携带kdr抗性基因型,抗性品系均携带kdr抗性基因型。抗性品系抗性杂合子占比56%,纯合子占比44%。这提示在高效氯氰菊酯的选择压力下,德国小蠊抗性的增加可能与其解毒酶活性的提高和kdr抗性基因型选择有关。The resistance of Blattella germanica to beta-cypermethrin was screened in laboratory to explore the resistant mechanism.After 10 generations of selection,Blattella germanica developed 8 fold resistance to beta-cypermethrin.Biochemical assay showed that the activity of carboxylesterase(CarE),glutathione S-transferase(GSTs)and cytochrome P450(CYP450)in resistant strains were significantly higher than those in susceptible ones.The specific activity of CarE,GSTs and CYP450 in resistant strains were 13.85 nmol/(min·mg),7.32 nmol/(min·mg)and 0.15 nmol/(min·mg)respectively,which appear higher than susceptible strains(6.85 nmol/(min·mg),2.71 nmol/(min·mg)and 0.08 nmol/(min·mg)).The knockdown resistance(kdr)genotype was determined by sequencing and the results showed that none of the susceptible strains had the kdr genotype,but all the resistant strains had the kdr genotype.Among the resistant strains,the heterozygotes genotype and the homozygotes genotype accounted for 56%and 44%respectively,implying that due to the selection pressure of beta-cypermethrin,the resistance of B.germanica may be related to the increased activity of detoxification enzyme and positive selection of kdr genotypes.

关 键 词：德国小蠊 高效氯氰菊酯 生化抗性 击倒抗性 

分 类 号：R184.3[医药卫生—流行病学]