miR-214在肥胖小鼠软骨损伤修复中的作用研究  

Role of miR-214 in the Repair of Cartilage Injury in Obese Mice

作　　者：曹红 吴伟[1] 周绪昌 邹军[1] 刘向云[1] 王淼[1] CAO Hong;WU Wei;ZHOU Xuchang;ZOU Jun;LIU Xiangyun;WANG Miao(School of Kinesiology,Shanghai University of Sport,Shanghai 200438,China)

机构地区：[1]上海体育学院运动科学学院,上海200438

出　　处：《中国细胞生物学学报》2020年第9期1578-1587,共10页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81871835、81772429);上海市人类运动能力开发与保障重点实验室(上海体育学院)(批准号:11DZ2261100)资助的课题。

摘　　要：该研究探究了miR-214在肥胖小鼠软骨损伤修复中的作用机制。从小鼠5周龄开始,进行为期20周的对照组(C组)普通饲料喂养,以及肥胖组(O组)与肥胖+运动组(OE组)高脂饲料喂养,当小鼠17周龄时,对OE组行8周的跑台运动,并于干预结束48 h后取材。饲养期间每周测量体质量,取材后行Micro-CT扫描、石蜡切片染色评分、RT-PCR。结果表明,O组、OE组小鼠体质量显著高于C组,跑台期间OE组较O组体质量呈下降趋势;O组染色评分显著高于C组,OE组Mankin’s评分较O组有显著性降低;O组胫骨平台内侧骨密度相关参数显著低于C组,OE组同等部位Tb.N显著高于O组,然而仅O组的胫骨平台外侧骨密度较C组显著降低;O组中炎症分解指标IL-6、ADAMTS5表达增高,合成指标COL2表达下降,同时miR-214表达增加,较C组均具有显著差异,OE组经过跑台干预后一定程度逆转了O组上述基因的表达情况。研究得出,跑台运动可抑制miR-214的表达,进而降低肥胖小鼠的软骨损伤退变风险,抑制炎症表达,促进软骨修复。This study explored the mechanism of miR-214 in the cartilage repair in obese mice.At the age of five weeks,the mice were divided into control group(C group),obesity group(O group)and obesity+exercise group(OE group).C group was fed with conventional diet,and O group and OE group were fed with high-fat diet for 20 weeks.At the age of 17 weeks,the OE group was given with treadmill exercise for eight weeks,and all the samples were taken 48 h after the last intervention.The body mass was measured every week during the feeding period.Micro-CT scanning,paraffin section staining score and RT-PCR were performed.The results showed that the body masses of O group and OE group were significantly higher than that of C group,and the body mass of OE group decreased compared with O group during treadmill running.The staining scores of O group were significantly higher than those of C group,while Mankin’s score of OE group was significantly lower than that of O group.The BMD-related parameters of the medial tibial plateau in O group were significantly lower than those in C group,and Tb.N at the same site in OE group was significantly higher than that in O group.However,on the outside of the tibial plateau,only the BMD of O group was significantly lower than that of C group.Compared with C group,the expression of IL-6,ADAMTS5,and miR-214 were increased,while COL2 was decreased in O group.The expression of these genes in O group could be partially reversed in OE group after treadmill exercise.Studies have concluded that treadmill exercise can inhibit the expression of miR-214,thereby reducing the risk of cartilage damage and degeneration in obese mice,inhibiting the expression of inflammation,and promoting cartilage repair.

关 键 词：肥胖 骨关节炎 软骨损伤 跑台运动 miR-214 

分 类 号：R684.3[医药卫生—骨科学]