毡毛马兰酚性成分及其抗肿瘤活性  

Phenolic constituents from Kalimeris shimadae and their antitumor activity

作　　者：黄宇飞 张楠 周忠玉 杨家欣 刘劲松 王国凯 HUANG Yu-fei;ZHANG Nan;ZHOU Zhong-yu;YANG Jia-xing;LIU Jin-song;WANG Guo-kai(Anhui Key Laboratory of Research Development of Chinese Medicine,School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Deparment of Medicinal Chemistry,Anhui Academy of Chinese Medicine,Hefei 230012,China;Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China;Department of Medical Research,China Medical University Hospital,China Medical University,Taichung 40447,China)

机构地区：[1]安徽中医药大学药学院,中药与开发安徽省重点实验室,安徽合肥230012 [2]安徽省中医药科学院药物化学研究所,安徽合肥230012 [3]中国科学院华南植物园,中国科学院植物资源保护与可持续利用重点实验室,广东广州510650 [4]台湾中国医药大学附设医院医学研究部,台湾台中40447

出　　处：《中成药》2020年第11期2922-2926,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81303220);中国科学院植物资源保护与可持续利用重点实验室开放基金(PCU201804);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2019035)。

摘　　要：目的研究毡毛马兰Kalimeris shimadae(Kitam.)Kitam.的酚性成分及其抗肿瘤活性。方法毡毛马兰乙醇提取物采用硅胶、凝胶及制备液相等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。MTT法测定其抗肿瘤活性。结果从中分离得到12个酚性化合物,分别鉴定为香草醛(1)、丁香醛(2)、阿魏酸(3)、2-(4-羟基-3-甲氧苯基)-3-(2-羟基-5-甲氧苯基)-3-氧-1-丙醇(4)、金色酰胺醇乙酸酯(5)、对羟基苯甲酸(6)、丁香酸(7)、对羟基苯甲醛(8)、3-羟基-1-(4-羟基-3,5-二甲氧苯基)-1-丙酮(9)、对羟基苯乙醇(10)、2,3-二羟基-1-(4-羟基-3,5-二甲氧苯基)-1-丙酮(11)、5,7-二羟基-4′,6,8-三甲氧基黄酮(12)。化合物6、8对8种细胞有一定的抑制作用。结论所有化合物均为首次从该植物中分离得到。化合物6、8有一定的细胞毒活性。AIM To study the phenolic constituents from Kalimeris shimadae(Kitam.)Kitam.and their anti-tumor activity.METHODS The ethanol extract from K.shimadae was isolated and purified by silica,Sephadex LH-20 and pre-HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their antitumor activities were evaluated by MTT.RESULTS Twelve phenolic compounds were isolated and identified as 3-methoxy-4-hydroxybenzaldehyde(1),syringaldehyde(2),ferulic acid(3),2-(4-hydroxy-3-methoxyphenyl)-3-(2-hydroxy-5-methoxyphenyl)-3-oxo-1-propanol(4),aurantiamide acetate(5),p-hydroxybenzoic acid(6),syringic acid(7),p-hydroxy benzaldehyde(8),3-hydroxy-1-(4-hydroxy-3,5-dim-ethoxyphenyl)-1-propanone(9),p-hydroxy phenylethanol(10),2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxy-phenyl)-1-propanone(11),5,7-dihydroxy-4′,6,8-trimethoxyflavone(12).Compounds 6,8 had a certain in-hibitory effect on the eight cells.CONCLUSION All the compounds are isolated from the plant for the first time,and compunds 6 and 8 have a certain cytotoxic activities.

关 键 词：毡毛马兰 酚性成分 分离鉴定 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]