黄柏生品与盐炙品中生物碱类成分在大鼠肾组织脏器中的吸收差异  

Absorption differences between the raw and salt-water processed Phellodendron chinense in rats kidney

作　　者：张凡[1,2] 孟莉 刘蓬蓬[1,2] 王旭 王锐[1] 宋成 贾天柱 ZHANG Fan;MENG Li;LIU Peng-peng;WANG Xu;WANG Rui;SONG Cheng;JIA Tian-zhu(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Traditional Chinese Materia Medica Processing Engineering Center of Liaoning Province,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中成药》2020年第11期2954-2959,共6页Chinese Traditional Patent Medicine

基　　金：国家自然基金项目(81274083,8190140513);辽宁省自然基金项目(20180550942);辽宁中医药大学大学生创新创业项目(201910162044)。

摘　　要：目的探究黄柏生品与盐炙品中生物碱类成分在大鼠肾组织脏器中的吸收差异。方法在大鼠口服灌胃给药后,采用UPLC-QqQ-MS法测定黄柏生品与盐炙品中黄柏碱、木兰花碱、药根碱、巴马汀、小檗碱成分在肾组织脏器中的含药浓度。结果给药后相同时间内,盐炙后的黄柏在肾组织脏器中生物碱类成分的含药浓度要比生品的含药浓度高。结论盐炙后的黄柏肾脏吸收效果较生品好,并在一定程度验证了黄柏“盐炙入肾”的炮制传统理论。AIM To study the absorption differences between the raw and salt-water processed Phellodendron chinense Schneid.in rats kidney.METHODS UPLC-QqQ-MS method was used to determine the content of phel-lodendrine,magnoflorine,jatrorrhizine,palmatine,berberine in rats kidney between the products of raw and salt-water processed P.chinense after oral administration.RESULTS Within the same time after administration,the content of alkaloids in the kidney tissue organs of salt-water processed of P.chinense was higher than the raw prod-ucts.CONCLUSION The absorption effect of salt-water processed P.chinense is better than the raw ones,and to a certain extent,it verifies the theory of“entering into the kidney through salt-water processing”.

关 键 词：黄柏 中药炮制 生物碱类 UPLC-QqQ-MS 

分 类 号：R284.1[医药卫生—中药学]