大柴胡汤及其拆方对非酒精性脂肪肝病(NAFLD)大鼠模型“肝-肠轴”影响的形态学  

Morphological Observation on the Effect of Dachaihu Decoction and Its Decomposition on“Liver-intestinal Axis”in NAFLD Model Rats

作　　者：高誉珊[1] 张鑫蕾 吴梦瑶 周璐[1] 张淑静[1] 陈琳[1] 王敏[1] 孙燕[1] 郑丰杰[1] 李宇航[1] GAO Yushan;ZHANG Xinlei;WU Mengyao;ZHOU Lu;ZHANG Shujing;CHEN Lin;WANG Min;SUN Yan;ZHENG Fengjie;LI Yuhang(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《世界中医药》2020年第21期3260-3265,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(81673868)。

摘　　要：目的:观察大柴胡汤及其拆方(方剂要素)对非酒精性脂肪肝病(NAFLD)大鼠模型“肝-肠轴”肝、肠形态学的影响。方法:采用随机数字表法将大鼠随机分为正常组、模型组、盐酸吡格列酮组、大柴胡汤组、疏肝利胆组、健脾化痰组、通腑泄浊组。采用高脂高糖饲料喂养16周建立NAFLD模型,并于第13~16周分别给予大柴胡汤及拆方。造模结束后,取小肠组织,电镜观察细胞间紧密连接情况,采用HE染色、油红O染色、Masson染色观察各组大鼠肝组织。结果:模型组肠细胞间隙明显增大,紧密连接程度降低,各给药组细胞间隙均有不同程度地减小,以全方组最为显著;正常组大鼠肝细胞结构正常,模型组大鼠部分肝细胞呈气球样变性,见不同程度脂滴形成,各给药组较模型组脂滴情况均有不同程度减轻。结论:大柴胡汤及其拆方均能不同程度地改善NAFLD模型大鼠肝细胞脂肪变及肠细胞紧密连接情况,并以全方共用效果尤著,疏肝利胆、健脾化痰、通腑泄浊等治法联合运用对改善NAFLD具有较好的疗效。Objective:To observe the effect of Dachaihu Decoction(DD)and its decomposition(formula-factors)on liver and intestine morphology of non-alcoholic fatty liver disease(NAFLD)model rats.Methods:Rats were divided into a normal group,a model group,a pioglitazone hydrochloride group,a DD group,a Shugan Lidan group,a Jianpi huatan group and a Tongfu Xiezhuo group by random number table.NAFLD model was established by feeding high-fat and high-sugar diet for 16 weeks,and DD and decomposition were given at 13-16 weeks respectively.After modeling,small intestinal tissues were taken and tight junctions between cells were observed by electron microscopy.Liver tissues of rats in each group were observed by HE,oil red O and Masson staining.Results:The intestinal intercellular space of the model group increased significantly and the degree of tight junction decreased,the intercellular space of each drug group decreased,especially in the DD group.The structure of hepatocytes in the normal group was normal,some hepatocytes in the model group showed balloon-like degeneration,and lipid droplets were formed in varying degrees.The lipid droplets in each drug group were alleviated to compare with the model group.Conclusion:DD and its decomposition can improve the fatty degeneration of hepatocytes and the tight junctions of intestinal cells in NAFLD model rats to varying degrees,and the effect of all prescriptions is particularly effective.The combined use of treatments such as soothing the liver and promoting gallbladder,invigorating the spleen and removing phlegm,and clearing the fu organs can improve NAFLD with good effect.

关 键 词：大柴胡汤 方剂要素 拆方研究 非酒精性脂肪性肝病 肝-肠轴 形态学 电镜 油红O染色 

分 类 号：R289.4[医药卫生—方剂学]