Metabolomic modulations in a freshwater microbial community exposed to the fungicide azoxystrobin  

作　　者：Mengwei Zhang Wanyue Liu Qian Qu Mingjing Ke Zhenyan Zhang Zhigao Zhou Tao Lu Haifeng Qian 

机构地区：[1]College of Environment,Zhejiang University of Technology,Hangzhou 310032,China [2]Department of Jianhu,Zhejiang Industry Polytechnic College,Shaoxing 31200,China [3]Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China

出　　处：《Journal of Environmental Sciences》2020年第11期102-109,共8页环境科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2017YFD0200503);the National Natural Science Foundation of China(Nos.21777144,21976161);the Changjiang Scholars and Innovative Re-search Team in University(No.IRT_17R97)。

摘　　要：An effective broad-spectrum fungicide,azoxystrobin(AZ),has been widely detected in aquatic ecosystems,potentially affecting the growth of aquatic microorganisms.In the present study,the eukaryotic alga Monoraphidium sp.and the cyanobacterium Pseudanabaena sp.were exposed to AZ for 7 days.Our results showed that 0.2–0.5 mg/L concentrations of AZ slightly inhibited the growth of Monoraphidium sp.but stimulated Pseudanabaena sp.growth.Meanwhile,AZ treatment effectively increased the secretion of total organic carbon(TOC)in the culture media of the two species,and this phenomenon was also found in a freshwater microcosm experiment(containing the natural microbial community).We attempted to assess the effect of AZ on the function of aquatic microbial communities through metabolomic analysis and further explore the potential risks of this compound.The metabonomic profiles of the microcosm indicated that the most varied metabolites after AZ treatment were related to the citrate cycle(TCA),fatty acid biosynthesis and purine metabolism.We thereby inferred that the microbial community increased extracellular secretions by adjusting metabolic pathways,which might be a stress response to reduce AZ toxicity.Our results provide an important theoretical basis for further study of fungicide stress responses in aquatic microcosm microbial communities,as well as a good start for further explorations of AZ detoxification mechanisms,which will be valuable for the evaluation of AZ environmental risk.

关 键 词：AZOXYSTROBIN Cyanobacterial bloom Microalgae Aquatic microbial community metabolomics analyses 

分 类 号：X524[环境科学与工程—环境工程]