嗜麦芽窄食单胞菌随机扩增多态性DNA基因分型及同源性分析  

Randomly amplified polymorphic DNA genotyping and homology analysis of Stenotrophomonas maltophilia

作　　者：朱雪峰 刘娟 赵苏瑛[3] 吴倩[2] ZHU Xue-feng;LIU Juan;ZHAO Su-ying;WU Qian(Safety Asessment and Research Center for Drug,Pesticide and Veterinary Drug of Jiangsu Province,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China;不详)

机构地区：[1]南京医科大学公共卫生学院江苏省医药农药兽药安全性评价与研究中心,江苏南京211166 [2]南京医科大学公共卫生学院卫生检验学系,江苏南京211166 [3]南京中医药大学附属医院检验科,江苏南京210029

出　　处：《中国卫生检验杂志》2020年第20期2439-2441,共3页Chinese Journal of Health Laboratory Technology

基　　金：江苏高校品牌专业建设工程资助项目(PPZY2015-A067);江苏高校优势学科建设工程资助项目。

摘　　要：目的采用一种快速,准确的基因分型方法,针对嗜麦芽窄食单胞菌导致的医院内感染进行流行病学调查,为预防院内交叉感染提供理论依据。方法收集南京市某医院住院患者痰液样本,并分离嗜麦芽窄食单胞菌,采用随机扩增多态性DNA(RAPD)技术获得指纹图谱,并利用SAS9.2软件对其进行聚类分析。结果 39株嗜麦芽窄食单胞菌菌株根据其DNA条带聚类分析,可分为A、B、C、D、E、F和G 7个型,A、B、D、E均有2个亚型,其中A型为优势型别。同源性分析结果表明在神经内科与呼吸内科,ICU与呼吸内科分别有同一克隆株的传播,科室之间可能存在交叉感染。结论 RAPD是一种简单快速,低成本的基因分型方法,适合临床病原菌的分型及医院感染分子流行病学研究。Objective To establish a rapid and accurate genotyping method for epidemiological investigation of Stenotroph-omonasmaltophilia,and to provide a scientific basis for the prevention of nosocomial infection.Methods Sputum samples of inpatients from a hospital in Nanjing was collected to isolate Streptomonas maltophilia,random amplified polymorphic DNA(RAPD)technology was used to obtain fingerprints,and SAS9.2 statistical software were conducted for cluster analysis.Results According to the result of the cluster analysis,all the 39 strains were divided to 7 clones,A,B,C,D,E,F and G.The type A wasthedominant clone.Theresultsindicatedthat there was a same clone spreading between the departments of neurology and respiration,so were the ICU and respirationdepartment.Conclusion The RAPD technique used in this study is simple and rapid for genotyping of S.maltophilia.It is valuable in the molecular repidemiological investigation of nosocomial infection.

关 键 词：嗜麦芽窄食单胞菌 随机扩增多态性DNA技术 基因分型 同源性 

分 类 号：R446.5[医药卫生—诊断学]