Digging out molecular markers associated with low salinity tolerance of Nannochloropsis oceanica through bulked mutant analysis  

作　　者：LIANG Sijie ZHANG Zhongyi LIU Hang GUO Li SUN Shiyang YANG Guanpin 

机构地区：[1]College of Marine Life Sciences,Ocean University of China(OUC),Qingdao 266003,China [2]Key Laboratory of Marine Genetics and Breeding of Ministry of Education,OUC,Qingdao 266003,China [3]College of Fisheries,OUC,Qingdao 266003,China [4]Institute of Evolution and Marine Biodiversity,OUC,Qingdao 266003,China

出　　处：《Journal of Oceanology and Limnology》2020年第6期1867-1879,共13页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(Nos.2018YFD0900305,2018YFD0901506);the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0406-3);the Fundamental Research Funds for the Central Universities(No.201762017)。

摘　　要：Nannochloropsis oceanica is a marine microalgal species with both economic value and biological importance.It grows fast,contains rich oils,reproduces asexually,holds a small and haploidy genome,and is easy to be modified genetically.However,the genetic study of N.oceanica is scarce.Very less genetic bases of its traits have been deciphered,and no gene has been isolated from it with the function verified simultaneously via either genetic or reverse genetic approaches or both(de novo cloned).Changing medium salinity may aid to control harmful organisms met during large scale cultivation.As a stress,it may also facilitate the accumulation of desirable chemicals including fatty acids.In order to decipher the genetic basis of the low salinity tolerance of N.oceanica,we mutated N.oceanica with Zeocin.In total,five mutant bulks were constructed at equal number of cells,100 mutants each,which were tolerant to a discontinuous serial of salinities from that of 100%of f/2 to that of a mixture of 4%of f/2 and 94%of BG11.The bulks were genotyped through whole genome re-sequencing and analyzed with bulked mutant analysis(BMA)newly modified from bulked segregant analysis(BSA).In total,47 SNPs and 112 InDels were found to associate with the low salinity tolerance,and around them a set of low salinity tolerance associating genes were identified.A set of annotatable genes commonly found between control and different salinities indicated that the genes functioning in gene expression,energy metabolism and cellular structure may be involved in the low salinity tolerance.These associating molecular markers and genes around them were not enough for outlining the physiological mechanism underlining the tolerance;however they should aid to improve N.oceanica genetically.

关 键 词：Nannochloropsis oceanica bulked mutant analysis(BMA) low salinity tolerance 

分 类 号：Q943.2[生物学—植物学]