黑曲霉发酵液和过氧化钙协同强化黑麦草提取土壤中铀  

Synergistic Enhancement of Phytoextraction of Uranium Using Lolium Perenne L.from Soil by Aspergillus Niger Fermentation Broth and Calcium Peroxide

作　　者：丁志超 胡南[1,2] 朱若男 马静 孙静[1,2] 李广悦[1,2] DING Zhichao;HU Nan;ZHU Ruonan;MA Jing;SUN Jing;LI Guangyue(Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang 421001,China;Hunan Province Key Laboratory of Green Development Technology for Extremely Low Grade Uranium Resources,Hengyang 421001,China)

机构地区：[1]南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳421001 [2]南华大学极贫铀资源绿色开发技术湖南省重点实验室,湖南衡阳421001

出　　处：《有色金属工程》2020年第11期129-136,共8页Nonferrous Metals Engineering

基　　金：湖南省中央引导地方科技发展专项资金项目(2018CT5009,YDZX201843000028582018)。

摘　　要：以黑麦草为供试植物,通过温室大棚实验,开展铀表土植物提铀研究。研究CaO2、黑曲霉发酵液及两者协同施用对黑麦草部分生理特性及其强化提铀能力的影响。结果表明,单独施加高剂量黑曲霉发酵液可促进黑麦草生长,但单独施加高剂量CaO2及协同施用高剂量CaO2+发酵液均对黑麦草生长有抑制。所有处理组都对黑麦草叶片可溶性蛋白积累有促进作用,其中单独施加40 mL/kg发酵液时,可溶性蛋白含量最高,比对照组相增加了511.11%。单独施加黑曲霉发酵液使黑麦草叶片SOD活性和MDA含量降低,而单独施加高剂量CaO2和协同施加高剂量CaO2+发酵液可使SOD活性和MDA含量增加。所有处理组均提高了土壤溶液铀浓度和黑麦草富铀能力,其中1 g/kg CaO2+20 mL/kg发酵液协同施用时效果最佳,黑麦草地上部分灰分中铀品位增加了622.80%,对铀的富集量提高了483.65%。Greenhouse soil culture experiments were conducted to investigate the phytoextraction of uranium from topsoil by Lolium perenne L.The effects of addition of CaO2,Aspergillus niger fermentation broth and their combination on some of physiological characteristics of Lolium perenne L.and enhancement of uranium phytoextraction were investigated.The results showed that the separate application of high dosed A.niger fermentation broth had a stimulative effect on Lolium perenne L.growth whereas the separate application of high dosed CaO2,the joint application of high dosed CaO2 and A.niger fermentation broth both gave an inhibitive effect.Furthermore,all the treatments resulted in an increased accumulation of soluble protein in Lolium perenne L.leaves,among which the separate addition of 40 mL/kg fermentation broth generated the highest content,increasing by 511.11%compared to the control.The application of A.niger fermentation alone led to the decreases in superoxide dismutase(SOD)activity and malondialdehyde(MDA)contents in Lolium perenne L.leaves,while the separate application of high dosed CaO2,joint application of high dosed CaO2 and A.niger fermentation broth both caused a reveres effect.Moreover,the uranium concentration in the soil solution and uranium phytoextraction raised in all the treatments,in which the combined application at 1 g/kg CaO2 and 20 mL/kg fermentation broth showed the best effect;uranium content in the ash of Lolium perenne L.above ground increased by 622.80%,and the total accumulation of uranium increased by 483.65%against the control.

关 键 词：铀 黑麦草 植物提取 强化 

分 类 号：X53[环境科学与工程—环境工程]