黄孢原毛平革菌降解磷酸三苯酯的性能和机理  

The performance and mechanism of triphenyl phosphate biodegradation by Phanerochaete chrysosporium.

作　　者：封觅 周家华 张军[2] 杨苑钰 郭育硕 FENG Mi;ZHOU Jia-hua;ZHANG Jun;YANG Yuan-yu;GUO Yu-shuo(National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China,Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management,Institute of Eco-environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou 510650,China;College of Environmental Science and Engineering,Guilin University of Technology,Guilin 541004,China;School of Environment and Energy,South China University of Technology,Guangzhou 510006,China)

机构地区：[1]广东省科学院生态环境与土壤研究所,华南土壤污染控制与修复国家地方联合工程研究中心,广东省农业环境综合治理重点实验室,广东广州510650 [2]桂林理工大学环境科学与工程学院,广西桂林541004 [3]华南理工大学环境与能源学院,广东广州510006

出　　处：《中国环境科学》2020年第11期4919-4926,共8页China Environmental Science

基　　金：广西科技基地和人才专项(桂科AD19245009);中国博士后科学基金资助项目(2019M662824);桂林理工大学科研启动基金资助项目(GUTQDJJ2018048);广东省科学院实施创新驱动发展能力建设专项(2020GDASYL-20200103076)。

摘　　要：以白腐真菌的中的黄孢原毛平革菌(Phanerochaete chrysosporium)为实验菌种,考察不同环境条件因素对P.chrysosporium降解磷酸三苯酯(TPhP)的影响以及TPhP降解过程中菌体细胞特性的变化.结果表明,当孢子液的接种量为4%(V/V),葡萄糖浓度为5g/L,pH值为5~6时,经过6d的处理P.chrysosporium对5mg/L TPhP的降解率可达到60%以上.菌体的细胞色素P450酶在TPhP的降解转化过程中发挥了重要的作用,抑制P450酶的活性会导致TPhP降解率下降.在降解反应的前期,为加快TPhP的代谢转化,菌体胞内蛋白含量以及ATP酶活性出现明显升高.在TPhP胁迫下,菌体SOD和CAT的活性随降解反应的进行呈现先升高后降低的趋势,2种抗氧化酶协同作用维持TPhP降解过程中菌体胞内的氧化还原平衡.Phanerochaete chrysosporium,one typical species of white rot fungi,was chosen as the experimental strains to investigate the effect of environmental condition on TPhP biodegradation by P.chrysosporium and the changes of cellular characteristics of P.chrysosporium during TPhP biodegradation.The results showed that the biodegradation efficiency of 5mg/L TPhP by P.chrysosporium was above 60%when inoculum size was 4%(V/V),pH was adjusted to 5~6 and initial glucose concentration was 5g/L.Cytochrome P450 of P.chrysosporium played an important role in TPhP biodegradation,the inhibition of cytochrome P450 could result in the decrease of TPhP biodegradation efficiency.In the earlier stage of TPhP biodegradation,the intracellular protein content and ATPase activity of P.chrysosporium increased remarkably to promote the metabolism and biotransformation of TPhP.Under the stress of TPhP,SOD and CAT activities of P.chrysosporium increased firstly and then decreased.There was a synergistic action between these two antioxidant enzymes in maintaining the intracellular redox balance during TPhP biodegradation.

关 键 词：黄孢原毛平革菌 磷酸三苯酯 降解性能 细胞特性 

分 类 号：X703[环境科学与工程—环境工程]