籽用美洲南瓜白粉病病原菌生理小种鉴定及抗性遗传分析  

Identification of Powdery Mildew Physiological Race in Seed-used Pumpkin(Cucurbita pepo L.)and Genetic Analysis of Its Resistance

作　　者：李书颉 王萍[1] 杨永升 张立东[2] 刘琦 王静 王思明 叶英杰[2] LI Shujie;WANG Ping;YANG Yongsheng;ZHANG Lidong;LIU Qi;WANG Jing;WANG Siming;YE Yingjie(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010010,Inner Mongolia,China;Tongliao Academy of Agricultural Sciences,Tongliao 028015,Inner Mongolia,China;Tongliao Economic Crops Workstation,Tongliao 028000,Inner Mongolia,China;Agricultural Technology Extension Center of Baotou,Baotou 014010,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特010010 [2]内蒙古通辽市农业科学研究院,内蒙古通辽028015 [3]内蒙古通辽市经济作物工作站,内蒙古通辽028000 [4]包头市农业技术推广中心,内蒙古包头014010

出　　处：《中国蔬菜》2020年第11期72-79,共8页China Vegetables

基　　金：内蒙古农业大学动植物品种选育(培育)专项计划项目(YZGC2017018);内蒙古科技计划项目(20090707,20100704,20110711,20120212)。

摘　　要：利用形态学和分子生物学的方法对内蒙古不同地区籽用美洲南瓜白粉病病原菌种类进行鉴定,并采用国际通用的13个甜瓜生理小种鉴别寄主和鉴别标准体系,对不同地区白粉病菌的生理小种进行鉴定。对20份籽用美洲南瓜种质的抗病性进行鉴定,并采用苗期人工接种的方法对籽用美洲南瓜六世代群体进行抗性统计分析,开展白粉病抗性遗传研究。形态学鉴定结果表明,籽用美洲南瓜白粉病病菌具有成串的椭圆形分生孢子,具有纤维状体。系统发育树鉴定结果表明该白粉病菌ITS序列与GenBank中已公布的瓜类单囊壳白粉菌(Podosphaera xanthii)的序列聚在一个分枝,确定内蒙古地区白粉病菌为单囊壳白粉菌P.xanthii。初步确定内蒙古地区籽用美洲南瓜白粉病存在4个生理小种,分别为小种4、小种2F和小种1,还有1个未知的小种。种质抗性鉴定结果表明,20份籽用美洲南瓜种质中有7份抗病种质,4份中抗种质,1份高抗种质。内蒙古地区籽用美洲南瓜抗白粉病基因由1对主效隐性单基因控制。Identification was carried out on powdery mildew pathogenic species of seed-used pumpkin(Cucurbita pepo L.)from different regions of Inner Mongolia by morphology and molecular biology methods.The physiological race of powdery mildew from different regions were identified by using the international common used 13 different identification hosts of powdery mildew and identification system.The resistance of 20 seed-used pumpkin germplasms was evaluated,and statistical analysis on 6 generations progeny resistance was carried out using artificial inoculation method at the seedling stage.Studies on genetic resistance of powdery mildew was also carried out.The results of morphological identification indicated that the powdery mildew of seed-used pumpkin had bunchy ellipsoidal conidium having corpus fibronsum.The result of molecular identification showed that the rDNA-ITS sequence of powdery mildew was in a branch with the sequence of Podsphaera xanthii,published in GenBank.The powdery mildew in Inner Mongolia region was identified as P.xanthii,and 4 physiological races of powdery mildew identified in Inner Mongolia region were race 4,race 2F,race 1,and one unknown race.The test results of germplasm resistance showed that among the 20 seed-used pumpkin germplasms 7 were disease-resistant,4 with medium-resistance,and 1 with high-resistance.The powdery mildew resistance genes of seed-used pumpkin in Inner Mongolia region were controlled by a pair of major recessive single gene.

关 键 词：籽用美洲南瓜 白粉病 病原菌鉴定 生理小种 遗传分析 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]