注射型富血小板纤维蛋白联合BMSCs在坐骨神经损伤的实验研究  

Experimental study of injection platelet-rich fibrin combined with BMSCs on sciatic nerve injury

作　　者：叶放[1] 王彦生[1] 于宁[1] 许蕙[1] YE Fang;WANG Yan-sheng;YU Ning;XU Hui(The Fifth Department of Hand Surgery,Central Hospital Affiliated to Shenyang Medical College,Liaoning Province,Shenyang110000,China)

机构地区：[1]沈阳医学院附属中心医院手外5科,辽宁沈阳110000

出　　处：《中国当代医药》2020年第31期9-12,共4页China Modern Medicine

基　　金：沈阳医学院科技基金项目(20191012)。

摘　　要：目的探讨注射型富血小板纤维蛋白联合骨髓间充质干细胞(BMSCs)在坐骨神经损伤中的应用效果。方法选择10日龄SD乳鼠6只,雌雄不限,培养纯化BMSCs群备用;另选取2月龄SD大鼠24只,雌雄各半,随机分为观察组与对照组,各12只,以改良挤压损伤法制作大鼠坐骨神经损伤模型,观察组与损伤部位局部注射BMSCs悬液250μl及自体富血小板纤维蛋白250μl,对照组局部注射生理盐水500μl,治疗14 d后,比较治疗前后两组坐骨神经功能指数变化,治疗14 d后大鼠坐骨神经轴突透射电镜图像及腓肠肌湿质量变化情况。结果治疗前两组坐骨神经功能指数比较,差异无统计学意义(P>0.05);治疗后观察组坐骨神经功能指数低于同组治疗前及治疗后的对照组(P<0.05);治疗14 d后,大鼠坐骨神经透射电镜提示观察组髓鞘轴突比例高于对照组,观察组腓肠肌湿质量高于对照组,且观察组腓肠肌湿质量恢复率高于对照组,差异有统计学意义(P<0.05)。结论针对坐骨神经损伤大鼠,使用自体注射型富血小板纤维蛋白联合BMSCs进行治疗,能有效提高坐骨神经功能,促进神经修复,提高平滑肌与血管再生能力。Objective To investigate the application effect of injection platelet-rich fibrin combined with Bone marrow mesenchymal stem cells(BMSCs)on sciatic nerve injury.Methods A total of 6 cases 10-day-old SD suckling rats of either sex were cultured and purify BMSCs group for later use,and 242-month-old SD rats half male and half female of either sex were selected,and 24 cases 2-month-old SD rats were randomly divided into observation group and control group,each had 12 cases.A rat sciatic nerve injury model was established by modified extrusion injury method.BMSCs suspension 250μl and autologous platelet-rich fibrin 250μl were locally injected into the observation group and the injury site,and 500μl of normal saline was locally injected into the control group.Then the changes of sciatic nerve function index before and after intervention were compared between the two groups.The result of transmission electron microscope images of sciatic nerve axons of rats were analyzed 14 days after treatment of the two groups.The changes of wet quality of gastrocnemius muscle in 14 days after intervention in the two groups were counted.Results Before treatment,there was no significant difference in sciatic nerve function index between the two groups(P>0.05);after treatment,the sciatic nerve function index of the observation group was lower than that before treatment and of the control group,the difference was statistically significant(P<0.05);after 14 days of treatment,the rat sciatic nerve transmission electron microscope showed that the ratio of myelin axons in the observation group was higher than that in the control group,the wet mass of the gastrocnemius in the observation group was higher than that in the control group,and the recovery rate of the wet mass of the gastrocnemius in the observation group was higher than that in the control group,the differences were statistically significant(P<0.05).Conclusion For sciatic nerve injury rats,the use of autologous injection platelet-rich fibrin combined with BMSCs for treatment c

关 键 词：注射型富血小板纤维蛋白 骨髓间充质干细胞 坐骨神经损伤 再生能力 

分 类 号：R965.1[医药卫生—药理学]