地骨皮正丁醇部位化学成分研究  

Chemical constituents from n-butanol parts of Lycii Cortex

作　　者：段雅迪 于潇 刘鹏[1] 陈晓鹤 姜艳艳[1] 张薇 刘斌[1] DUAN Ya-di;YU Xiao;LIU Peng;CHEN Xiao-he;JIANG Yan-yan;ZHANG Wei;LIU Bin(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中草药》2020年第20期5123-5127,共5页Chinese Traditional and Herbal Drugs

基　　金：中央高校基本科研业务费专项资金资助(2018-JYBZZ-XJSJJ007);北京中医药大学青年科学家培育计划(BUCM-2019-QNKXJC008)。

摘　　要：目的研究地骨皮Lycii Cortex(枸杞Lycium chinense根皮)的化学成分。方法地骨皮干燥药材用70%乙醇回流提取,所得浸膏依次用石油醚、醋酸乙酯和正丁醇萃取。采用硅胶、Sephadex LH-20、ODS柱色谱和半制备高效液相色谱等方法对地骨皮正丁醇萃取部位进行分离纯化,运用核磁、质谱等现代波谱技术对分离得到的化合物进行结构鉴定。结果从地骨皮的正丁醇萃取部位中分离得到10个化合物,分别鉴定为(1'S,2R,5S,10R)-2-(1',2'-二羟基-1'-甲基)-6,10-二甲基螺[4,5]癸-6-烯-8-酮-2'-O-β-D-葡萄糖苷(1)、(1'R,2R,5S,10R)-2-(1',2'-二羟基-1'-甲基-)-6,10-二甲基螺[4,5]癸-6-烯-8-酮-2'-O-β-D-葡萄糖苷(2)、(1R,6R,9S)-6,9,11-三羟基大柱香波龙-4,7-二烯-3-酮-11-O-β-D-葡萄糖苷(3)、香草酸-4-O-β-D-葡萄糖苷(4)、3,4-二羟基苯丙酸(5)、3,4-二羟基苯丙酸甲酯(6)、丁香酸葡萄糖苷(7)、二氢红花菜豆酸-3'-O-β-D-吡喃葡萄糖苷(8)、异东莨菪苷(9)和fabiatrin(10)。结论化合物3为首次从茄科植物中分离得到,化合物1、2、4为首次从枸杞属植物中分离得到,且化合物2的核磁数据首次被报道。Objective To study the chemical constituents from n-butanol-souluble part of Lycii Cortex(the root bark of Lycium chinense).Methods The air-dried Lycii Cortex were powdered and extracted with 70%ethanol under reflux.After the removal of solvent under reduced pressure,the crude extract was extracted with petroleum ether,ethyl acetate and n-butanol successively.The compounds were isolated and purified by silica gel,Sephadex LH-20,ODS and semi-prepared high performance liquid chromatography from the n-butanol part of Lycii Cortex.The structures were identified by nuclear magnetic spectrometry,mass spectrometry and other spectral analyses.Results Ten compounds were isolated from n-butanol parts of Lycii Cortex and characterized as(1'S,2R,5S,10R)-2-(1',2'-dihydroxy-1'-methylethyl)-6,10-dimethylspiro[4,5]dec-6-en-8-one 2'-O-β-D-glucopyranoside(1),(1'R,2R,5S,10R)-2-(1',2'-dihydroxy-1'-methylethyl)-6,10-dimethylspiro[4,5]dec-6-en-8-one 2'-O-β-D-glucopyranoside(2),(1R,6R,9S)-6,9,11-trihydroxy-4,7-megastigmadien-3-one 11-O-β-D-glucopyranoside(3),vanillic acid-4-O-β-D-glucopyranoside(4),3,4-dihydroxyphenylpropionic acid(5),3,4-dihydroxybenzenepropionic acid methyl ester(6),glucosyringic acid(7),dihydrophaseic acid 3'-O-β-D-glucopyranoside(8),isoscoploletin-β-D-glucoside(9)and fabiatrin(10).Conclusion Compound 3 is isolated from Solanaceae family for the first time and compounds 1,2 and 4 are isolated from Lycium genus for the first time.The NMR data of compound 2 is first reported as well.

关 键 词：地骨皮 枸杞 R 2R 5S 10R)-2-(1' 2'-二羟基-1'-甲基-)-6 10-二甲基螺[4 5]癸-6-烯-8-酮-2'-O-β-D-葡萄糖苷 (1R 6R 9S)-6 9 11-三羟基大柱香波龙-4 7-二烯-3-酮-11-O-β-D-葡萄糖苷 香草酸-4-O-β-D-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]