TPPA和TRUST试验在梅毒诊断中的应用  

Application of TPPA and TRUST test in the diagnosis of syphilis

作　　者：郑维威 崔迪[2] 梅传忠[2] 张文静[1] ZHENG Wei-wei;CUI Di;MEI Chuan-zhong;ZHANG Wen-jing(First Affiliated Hospital of University of Science and Technology of China,Hefei,Anhui 230001,China;不详)

机构地区：[1]中国科学技术大学附属第一医院,安徽合肥230001 [2]蚌埠医学院

出　　处：《中国国境卫生检疫杂志》2020年第4期276-277,共2页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的评价梅毒螺旋体明胶颗粒凝集试验(TPPA)和甲苯胺红不加热血清试验(TRUST)在梅毒患者检测中的临床应用。方法2018年12月—2019年6月中国科学技术大学附属第一医院疑似梅毒的住院患者319例,取血清样本,分别用TPPA、TRUST两种方法检测梅毒螺旋体抗体,分析其检测结果。结果319份样本中,TPPA法阳性率为96.24%,TRUST法阳性率为36.68%。以TPPA为标准,TRUST的灵敏度38.11%,特异性100.00%,阳性预测值100.00%,阴性预测值5.94%,阳性率明显低于TPPA法,差异有统计学意义(P<0.01)。结论TPPA快速、有效、特异性和灵敏度高,可用于确诊梅毒感染;TRUST试验则可作为梅毒患者疗效观察及复发监测的指标。Objective To evaluate the clinical application of Treponema pallidum gelatin particle agglutination test(TPPA)and toluidine red unheated serum test(TRUST)in the detection of syphilis casses.Methods From December 2018 to June 2019,319 suspected syphilis patients in the First Affiliated Hospital of University of Science and Technology of China were collected.Their serum samples were detected Treponema pallidum antibody by using TP-PA and TRUST methods.Results The positive rate of TPPA and TRUST was 96.24%and 36.68%,respectively.According to TPPA,the sensitivity,specificity,positive predictive value and negative predictive value of TRUST was 38.11%,100.00%,5.94%and 100.00%,respectively,which was significantly lower than those of TPPA(P<0.01).Conclusion TPPA was rapid,effective,specific and sensitive for serological detection of syphilis infection,and TRUST could be used as an index for efficacy observation and recurrence monitoring of syphilis patients.

关 键 词：梅毒 甲苯胺红不加热血清试验 明胶凝集试验 

分 类 号：R446.5[医药卫生—诊断学]