水热预处理与Myceliophthora thermophila复合酶协同高效降解混合生物质的研究  

High Efficient Degradation of Mixed Biomass by Hot-Water Pretreatment and Synergistic Enzymatic Hydrolysis between Myceliophthora thermophila Composite Enzyme and Cellulase

作　　者：钱玉霞 邱卫华[2,3] 任虹 QIAN Yuxia;QIU Weihua;REN Hong(School of Light Industry,Beijing Laboratory of Food Quality and Safety,Beijing Technology and Business University,Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry,Beijing 100048,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;Dalian National Laboratory for Clean Energy,Chinese Academy of Sciences,Dalian 116023,China)

机构地区：[1]北京工商大学轻工科学技术学院,食品质量与安全北京实验室,中国轻工业清洁生产和资源综合利用重点实验室,北京100048 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]中国科学院洁净能源创新研究院,辽宁大连116023

出　　处：《化学与生物工程》2020年第11期34-41,共8页Chemistry & Bioengineering

基　　金：中国科学院洁净能源研究院合作基金项目(DNL180305);2020年实培计划——大学生科研训练计划深化项目。

摘　　要：以甘蔗渣-棉杆-小麦秸秆(4∶2∶1,质量比)混合生物质为研究对象,考察了水热预处理参数对混合生物质中各组分含量的影响,研究了嗜热毁丝霉(Myceliophthora thermophila)复合酶(MTE)与纤维素酶(Cel)协同作用下水热预处理混合生物质的酶解效率,并对酶解液及酶解渣分别进行UV、FTIR分析。结果表明,水热预处理温度是影响混合生物质中各组分含量变化的主要因素,在相近水热预处理强度下,经150℃维温30 min和180℃维温5 min处理的混合生物质中木质素含量分别为(26.50±1.33)%和(29.40±1.35)%。MTE对Cel具有明显的协同作用,且受水热预处理温度的影响较大。MTE对经160℃维温10 min处理的混合生物质的Cel酶解协同效率最高达(53.60±2.68)%,酶解总糖得率达(75.50±3.77)%;而继续升高温度或者延长维温时间,虽然酶解总糖得率略有升高,但MTE的协同酶解效率快速降低。UV和FTIR分析表明,MTE所含的木质纤维素分解酶和木质素氧化酶可在较低预处理强度下协同Cel提高混合生物质的酶解效率。Using bagasse-cotton stalk-wheat straw(4∶2∶1,mass ratio)mixed biomass as the research object,we investigated the effects of hot-water pretreatment parameters on the content of each component in the mixed biomass,studied the enzymatic hydrolysis efficiency of the mixed biomass by synergistic enzymatic hydrolysis between Myceliophthora thermophila composite enzyme(MTE)and cellulase(Cel),and analyzed enzymolysis liquid and enzymolysis residue by UV and FTIR,respectively.The results show that the hot-water pretreatment temperature is the main factor affecting the content of each component in the mixed biomass.Under the same hot-water pretreatment intensity,the lignin content in the mixed biomass is(26.50±1.33)%and(29.40±1.35)%,respectively,after being pretreated at 150℃for 30 min and at 180℃for 5 min,respectively.MTE has obvious synergistic effect on Cel,which is greatly affected by hot-water pretreatment temperature.After being pretreated at 160℃for 10 min,the highest synergistic enzymatic hydrolysis efficiency can reach(53.60±2.68)%,and the total sugar yield can reach(75.50±3.77)%,by synergistic enzymatic hydrolysis between MTE and Cel.Further increase of the temperature and time can lead to the slight increase of the total sugar yield while the rapid decrease of synergistic enzymatic hydrolysis efficiency of MTE.UV and FTIR analyses show that the lignocellulose hydrolase and lignin oxidase contained in MTE can cooperate with Cel to improve the enzymatic hydrolysis efficiency of mixed biomass under low pretreatment intensity.

关 键 词：混合生物质 水热预处理 嗜热毁丝霉 协同酶解 

分 类 号：TQ353.1[化学工程] Q819[生物学—生物工程]