Wnt/β-catenin、BMP-2/Runx2/Osterix、OPG/RANKL、LGR4/RANKL通路的相关因子在绝经后骨质疏松性骨折中的表达  

Expression of factors associated with Wnt/β-catenin,BMP-2/Runx2/Osterix,OPG/RANKL,and LGR4/RANKL pathway in patients with postmenopausal osteoporotic fractures

作　　者：王斌 麦彩园[2] 谢胜德 曹燕明[3] 汪志中 李新旭 徐茂森 WANG Bin;MAI Caiyuan;XIE Shengde;CAO Yanming;WANG Zhizhong;LI Xinxu;XU Maosen(Department of Orthopedics, People’s Hospital of Sanshui, Foshan 528100;Department of Obstetrics, Guangdong Women and Children’s Hospital, Guangzhou 510010;Department of Orthopedics, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China)

机构地区：[1]佛山市三水区人民医院创伤骨科,广东佛山528100 [2]广东省妇幼保健院产科,广东广州510010 [3]广州医科大学附属第二医院骨科,广东广州510260

出　　处：《中国骨质疏松杂志》2020年第11期1577-1583,1658,共8页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81574002);广东省医学科学技术研究基金(A2020377);佛山市科技局科研立项(2018AB000595)。

摘　　要：目的通过检测绝经后骨质疏松性骨折患者(PMOPF组)和对照组中血清、骨组织中因子LRP5、β-catenin、Runx2、C-myc、Osterix、OPG、RANKL、LGR4水平的表达,分析相关的通路与PMOPF的相关性。方法选取胫骨骨折患者分为对照组、PMOPF组。RNAiso Plus法提取骨组织总RNA,RT-qPCR法检测各因子表达。对照组通过酶联免疫分析方法(ELISA)检测血清各因子水平;PMOPF组按采血时段分A^F组,ELISA检测各因子水平。比较对照组与PMOPF组、PMOPF组中各邻组之间的变化。结果(1)RT-qPCR法检测PMOPF组骨组织LRP5、β-catenin、Runx2、C-myc、Osterix、OPG、LGR4水平明显下降(P<0.05),RANKL水平明显上升(P<0.05)。(2)ELISA法检测PMOPF组中A^F组各因子血清水平LRP5、β-catenin、Runx2、C-myc、Osterix、OPG、LGR4水平明显下降(P<0.05),RANKL水平明显上升(P<0.05)。LRP5、Runx2在B组最低,β-catenin、C-myc在C组最低,RANKL在C组最高,Osterix在D组最低,OPG、LGR4在E组最低。结论Wnt/β-catenin、BMP-2/Runx2/Osterix、OPG/RANKL、LGR4/RANKL通路的相关因子与PMOPF发生关系密切。LRP5、Runx2在骨折3 d内水平降至最低,β-catenin、C-myc在骨折7 d内水平降至最低,反映了其在成骨阶段中的变化一致;Osterix在骨折14 d内水平降至最低,OPG、LGR4在骨折28 d内水平降至最低,可能与PMOPF短期内较难愈合有关;RANKL在骨折7 d内水平升至最高,可能与PMOPF后成骨有所增加有关。Objective The correlation between related pathways and postmenopausal osteoporotic fractures(PMOPF)is analyzed by detecting the expressions of LRP5,β-catenin,Runx2,C-myc,Osterix,OPG,RANKL,and LGR4 in the serum and bone tissues in PMOPF patients and in patients with non-osteoporosis fractures after menopause(control group).Methods The patients with tibial fractures were divided into control group and PMOPF group.Total RNA of the bone tissue was extracted using RNAiso Plus method.RT-qPCR method was used to detect the expression of each factor.The levels of serum factors were detected with ELISA method in the control group.PMOPF group was divided into A-F group according to the blood collection time interval.The changes between the control group and the PMOPF group and between the adjacent groups in the PMOPF group were compared.Results(1)The expressions of LRP5,β-catenin,Runx2,C-myc,Osterix,OPG,and LGR4 in PMOPF group were lower than those in control group(P<0.05)detected by RT-qPCR.The expression of RANKL increased significantly(P<0.05).(2)The serum levels of LRP5,β-catenin,Runx2,C-myc,Osterix,OPG,and LGR4 decreased significantly(P<0.05),and RANKL increased significantly(P<0.05),in the A-F group of PMOPF group compared with those in the control group detected with ELISA method.LRP5 and Runx2 were the lowest in Group B.β-catenin and C-myc were the lowest in Group C.RANKL was the highest in Group C.Osterix was the lowest in Group D.OPG and LGR4 were the lowest in Group E.Conclusion The related factors in Wnt/β-catenin,BMP-2/Runx2/Osterix,OPG/RANKL,LGR4/RANKL pathways are closely related to the occurrence of PMOPF.LRP5 and Runx2 decreased to the lowest level within 3 days after fracture.β-catenin and C-myc decreased to the lowest level within 7 days after fracture.The result show that the changes in osteogenesis are consistent.Osterix decreased to the lowest level within 14 days after fracture.OPG and LGR4 decreased to the lowest level within 28 days after fracture,which might be related to the difficulty of sh

关 键 词：PMOPF Wnt/β-catenin通路 BMP-2/Runx2/Osterix通路 OPG/RANKL通路 LGR4/RANKL通路 

分 类 号：R683[医药卫生—骨科学]