葛根素通过OPG/RANKL及Wnt/β-catenin信号通路介导对来曲唑引起骨流失的保护作用  

Puerarin protects bone loss caused by letrozole via OPG/RANKL and Wnt/β-catenin signaling pathway

作　　者：韩俊[1] 蒋现永 叶恒[1] 陈东 HAN Jun;JIANG Xianyong;YE Heng;CHEN Dong(Department of Orthopedics, Hanyang Hospital, Wuhan University of Science and Technology, Wuhan 430050, China)

机构地区：[1]武汉科技大学附属汉阳医院骨科,湖北武汉430050

出　　处：《中国骨质疏松杂志》2020年第11期1604-1608,共5页Chinese Journal of Osteoporosis

基　　金：湖北省卫生健康委员会科研项目(WJ2019M038)。

摘　　要：目的观察葛根素对来曲唑治疗的老年雌性大鼠骨量和骨密度影响,并探索可能的机制。方法30只24个月龄老年雌性SD大鼠随机分为3组:对照组(CON组);来曲唑组(LQC组):每周给予0.1 mg/kg来曲唑治疗;葛根素+来曲唑组(GGS+LQC组):每天给予50 mg/kg葛根素联合每周给予0.1 mg/kg来曲唑治疗,为期12周,待治疗结束后使用Micro-CT、Masson染色切片、血清学检测以及蛋白质印迹观察治疗效果以及可能的机制。结果治疗12周后,与LQC组相比,GGS+LQC组的大鼠骨小梁数量和骨密度得到明显改善。GGS+LQC组大鼠BMD、BV/TV、Tb.N、Tb.Th和Tb.Sp较CON组明显改善(P<0.05)。治疗12周时,GGS+LQC组CTX-1和PINP水平较LQC组显著降低(P<0.05),比较差异有统计学意义(P<0.05)。和LQC组比较,GGS+LQC组的大鼠OPG/RANKL及Wnt/β-catenin信号通路被激活,OPG、Wnt3α、β-catenin水平显著上升,RANKL水平明显下降,比较差异有统计学意义(P<0.05)。结论葛根素通过激活OPG/RANKL及Wnt/β-catenin信号通路逆转来曲唑对老年雌性大鼠骨骼有害作用。Objective To observe the effect of puerarin on bone mass and bone mineral density(BMD)on aged female rats treated with letrozole,and to explore the possible mechanism.Methods Thirty 24-month-old female SD rats were randomly divided into three groups:control group(CON group),letrozole group(LQC group),which was treated with 0.1 mg/kg letrozole every week,and Puerarin+letrozole group(GGS+LQC group),which was treated with 50 mg/kg puerarin per day combined with 0.1 mg/kg letrozole per week for 12 weeks.After treatment,micro-CT,Masson staining sections,serological detection,and Western blotting were used to observe the therapeutic effect and the possible mechanism.Results After 12 weeks of treatment,the number of bone trabeculae and BMD in GGS+LQC group were significantly improved compared with those in LQC group.BMD,BV/TV,Tb.N,Tb.Th,and Tb.Sp in GGS+LQC group were significantly better than those in CON group.After 12 weeks of treatment,the levels of CTX-1 and PINP in GGS+LQC group were significantly lower than those in LQC group(P<0.05).Compared with LQC group,OPG/RANKL and Wnt/β-catenin signal pathway were activated,OPG,Wnt3α,andβ-catenin levels were significantly increased,and RANKL level was significantly decreased in GGS+LQC group(P<0.05).Conclusion Puerarin reverses the harmful effects of letrozole on the bone of aged female rats by activating OPG/RANKL and Wnt/β-catenin signal pathway.

关 键 词：来曲唑 葛根素 OPG/RANKL信号通路 WNT/Β-CATENIN信号通路 骨密度 

分 类 号：R285.5[医药卫生—中药学]