基于RuSi@PEI纳米粒子构建分子印迹电化学发光传感器检测普萘洛尔  

RuSi@PEI Nanoparticles-based Molecularly Imprinted Electrochemiluminescence Sensors for Propranolol Detection

作　　者：戢凯伦 徐玮 王颖 熊成义 张修华 王升富[1] JI Kailun;XU Wei;WANG Ying;XIONG Chengyi;ZHANG Xiuhua;WANG Shengfu(Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules,College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062;Wuhan Institute Food and Cosmetic Control,Wuhan 430012)

机构地区：[1]湖北大学化学化工学院,有机功能分子合成与应用教育部重点实验室,湖北武汉430062 [2]武汉食品化妆品检验所,湖北武汉430012

出　　处：《分析科学学报》2020年第5期747-752,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.11674085)。

摘　　要：本文构建了一种新型的分子印迹电化学发光(MIP-ECL)生物传感器,可高灵敏、高选择性测定普萘洛尔(PRO)分子。钌硅纳米粒子(Ru Si NPs)与聚乙烯亚胺(PEI)通过静电吸附形成Ru Si@PEI复合纳米粒子,这种纳米复合结构缩短了电子转移距离,进而提高了电子转移效率,有效增强ECL信号。利用透射电镜(TEM)、扫描电镜(SEM)、紫外-可见(UV-Vis)光谱和ECL等方法对合成Ru Si@PEI纳米粒子进行表征。以该复合纳米材料作为ECL探针,在此基础上与PRO印迹的聚合物膜结合,构建MIP-ECL传感器。实验对Ru Si@PEI纳米粒子合成过程中Ru Si NPs与PEI的体积比,洗脱与孵育时间进行优化,在最优条件下记录PRO被洗脱与重新印迹到传感器上时ECL信号的差值,其差值与PRO浓度之间呈现良好的线性关系,检测范围为1.0×10^(-11)~5.0×10^(-9)mol/L,检测限(S/N=3)低至5.4×10^(-13)mol/L。此外传感器在人血清样品中加标回收率为93.8%~112.7%,说明了该MIP-ECL传感器在复杂生物样品的PRO检测中具有潜在应用价值。In this paper,a novel molecularly imprinted electrochemiluminescence(MIP-ECL) sensor with high sensitivity and selectivity was constructed for the determination of propranolol(PRO).RuSi NPs were combined with polyethyleneimine(PEI) to formed RuSi@PEI composite nanoparticles(RuSi@PEI NPs) by electrostatic adsorption.The RuSi@PEI NPs were characterized by transmission electron microscope,scanning electron microscope,and ultraviolet-visible spectra and ECL.The RuSi@PEI NPs were used as electrochemiluminescence probe,and combined with the PRO molecularly imprinted polymers(MIPs) to construct a novel ECL sensor.The volume ratio of RuSi NPs to PEI,the elution and incubation time of PRO were optimized.Through recording the ECL signal difference between eluted and re-imprinted under optimal conditions,there was a good linear relationship between the signal difference and the PRO concentrations.The linear range was 1.0×10-11-5.0×10-9 mol/L,and detection limit was 5.4×10-13 mol/L(S/N=3).The recovery rate of the sensor in human serum samples was 93.8%-112.7%.This indicated that the MIP-ECL sensor had great potential for the PRO detection in complex samples.

关 键 词：电化学发光 分子印迹技术 传感器 普萘洛尔 

分 类 号：O657.1[理学—分析化学]