基于非靶向代谢组学的沉水樟内源生根抑制物分析  

Analysis of Endogenous Rooting Inhibitors in Cinnamomum micranthum Based on Non-targeted Metabolomics Approach

作　　者：邹显花[1,2] 胡亚楠 孙雪莲 黄勇来 温素芸 吴文景 马祥庆[1,2] ZOU Xian-hua;HU Ya-nan;SUN Xue-lian;HUANG Yong-lai;WEN Su-yun;WU Wen-jing;MA Xiang-qing(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fujian Provincial Colleges and University Engineering Research Center of Plantation Sustainable Management,Fuzhou 350002,Fujian,China;Seedling Station of Jian'ou Forestry Bureau,Jian'ou 353100,Fujian,China;Nanya Forestry Station in Jian'ou,Jian'ou 353101,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]人工林可持续经营福建省高校工程研究中心,福建福州350002 [3]福建建瓯市林业局种苗站,南平建瓯353100 [4]福建建瓯市南雅林业站,南平建瓯353101

出　　处：《林业科学研究》2020年第5期86-96,共11页Forest Research

基　　金：福建省林业科学技术推广项目(闽林推[2016]SJ15号)。

摘　　要：[目的]探究沉水樟扦插生根的阻碍因子,为提高沉水樟的扦插生根率提供理论依据。[方法]本研究基于非靶向LC-MS检测技术,比较筛选沉水樟不同枝龄穗条及不同组织的差异内含物,并结合扦插试验验证不同枝龄穗条的扦插效果,探讨内源生根抑制物对沉水樟扦插生根的影响。[结果]沉水樟不同枝龄穗条木质部及韧皮部所含的差异内含物中,总体上表现为酚类及有机酸类物质的相对峰面积显著高于其他物质,其中,酚类物质的相对峰面积最大,且韧皮部的酚类物质显著高于木质部。不同枝龄的穗条比较发现,韧皮部与木质部的酚类与有机酸类物质总体上均表现为1年生穗条显著高于其他枝龄,且随枝龄增加呈逐渐减少的趋势。与之相反的是,沉水樟不同枝龄穗条中,1年生穗条的扦插效果最优,且扦插成活率随枝龄的增加而降低。[结论]沉水樟穗条中存在以酚类与酸类为主的生根抑制物,且主要分布在韧皮部。此外,内源生根抑制物对沉水樟扦插生根的抑制作用随枝龄的增加呈减弱趋势。[Objective] Low rooting rate has been the key factor limiting the cuttage raising of Cinnamomum micranthum(Hay.) Hay, an endangered species. The study aims at determining the factors hindering the rooting of C. micranthum cuttage, so as to provide a theoretical basis for improving the rooting rate of C. micranthum.[Method] Different inclusions in different tissues of cuttings with different ages of C. micranthum were screened out based on the non-targeted detection technology. At the same time, cuttage experiment was used to verify the survival rate of cuttage with different ages. The authors tried to determine the effect of endogenous rooting inhibitors on cuttage rooting of C. micranthum. [Result] The results showed that the relative peak area of phenols and organic acids in xylem and phloem of C. micranthum were significantly larger than that of other substances, among which the relative peak area of phenols was the largest. It also showed that phenols content in phloem were higher than that in xylem. In addition,the content of phenols and organic acids in xylem and phloem of one-year-old branch were significantly higher than that of other branches with different ages, and the content decreased with the increase of cuttage age. Conversely, the survival rate of 1-year-old cuttage were the highest, and it decreased with the increase of cuttage age.[Conclusion] The rooting inhibitors are mainly phenolic and acid in the cuttage of C. micranthum and mainly distributed in phloem. The effect of endogenous rooting inhibitors on the rooting of cuttage will decrease with the increase of age of cuttage.

关 键 词：沉水樟 非靶向 扦插 生根抑制物 生根率 

分 类 号：S792.23[农业科学—林木遗传育种]