金丝楸优良无性系遗传多样性和亲缘关系的AFLP分析  

AFLP Analysis of Catalpa bungei Superior Clones

作　　者：马庆国[1] 王治军 徐慧敏 李明昆 李慧民 张俊佩[1] 张建国[1] MA Qing-guo;WANG Zhi-jun;XU Hui-min;LI Ming-kun;LI Hui-min;ZHANG Jun-pei;ZHANG Jian-guo(State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;Luoyang Academy of Agriculture and Forestry Sciences,Luoyang 471000,Henan,China;Forestry Technical Guidance Station of Luoning County,Luoning 471700,Henan,China;Khotan Area Forestry and Grassland Bureau,Khotan 848000,Xinjiang,China;Kash Forestry Technology Extension Station,Kash 844000,Xinjiang,China)

机构地区：[1]林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091 [2]洛阳农林科学院,河南洛阳471000 [3]洛宁县林业技术指导站,河南洛阳471700 [4]和田地区林业和草原局,新疆和田848000 [5]喀什地区林业技术推广站,新疆喀什844000

出　　处：《林业科学研究》2020年第5期145-153,共9页Forest Research

基　　金：中国林业科学研究院林业研究所林木培育重点实验室专项资金项目(ZDRIF201703)。

摘　　要：[目的]为了加深对金丝楸(Catalpa bungei C.A.Mey.)优良无性系资源遗传背景的认识,[方法]本研究运用荧光扩增片段长度多态性(AFLP)技术,筛选10对Eco RⅠ+3/MseⅠ+3引物组合,以梓树属的梓树(C.ovata G.Don)、灰楸(C.fargesii Bur.)、光叶楸和‘豫楸1号’良种为对照,在基因组水平上检测定植于河南省洛宁县的75个金丝楸无性系的遗传变异。[结果]总体水平上,在获得的1177条可统计条带中,1130条呈多态性,多态性带百分率(PPBs)达96.01%;不同位点的有效等位基因数为1.00~1.99,平均为1.48±0.03;基因多样度为0.00~0.49,平均为0.28±0.01;Shannon信息指数为0.00~0.69,平均为0.43±0.02。当遗传相似系数为0.8277时可将供试样品分为8组,其中,71个无性系聚为一组,显示出金丝楸无性系遗传背景较狭窄。各无性系的遗传距离和地理距离呈极显著正相关,表明地理隔离对楸树遗传多样性有明显的影响。结合聚类分析结果,金丝楸无性系当中的63号与其他遗传距离最远,值得重点保护和推广。[结论]形态学性状观察和AFLP分子标记分析显示:金丝楸与普通楸树存在较显著的差异,因此,认为金丝楸的分类地位宜做更加深入的探讨。[Objective] To study the genetic background and the genetic diversity of ’Jinsiqiu’(a species of Catalpa bungei) germplasm. [Method] AFLP was conducted using 10 Eco RⅠ+3/MseⅠ+3 primer combinations to analyze75 elite clones of C. fargesii, C. ovata, Guangyeqiu(a species of C. bungei) and C. bungei var. ’Yuqiu 1’ as control.[Result] 1 177 AFLP fragments were obtained totally, in which 1 130 were polymorphic and the average percentage of polymorphic bands(PPBs) was 96.01%. On the overall level,the value of effective number of alleles(Ne) ranged from 1.00 to 1.99 with an average of 1.48±0.03;Nei’s gene diversity(H) ranged from 0.00 to 0.49 with an average of0.28±0.01 and Shannon’s information index(I) ranged from 0.00 to 0.69 with an average of 0.43±0.02. All the materials used in the study could be divided into 8 groups, among which group I contained 71 ’Jinsiqiu’ elite clones. It indicated a moderate level of genetic diversity and the genetic background may be quite narrow among the materials used in the study. The genetic distance and geographical distance of each clone showed a significant positive correlation, indicating that geographical isolation had a significant effect on the genetic diversity of Catalpa. Combined with the results of cluster analysis, a clone of ’Jinsiqiu’ showed the furthest genetic distance from the others, which was worthy of special protection and promotion. [Conclusion] Morphological and AFLP molecular marker evidences show that ’Jinsiqiu’ is significantly different from the common C. bungei species. The authors suggest that the taxonomic position of ’Jinsiqiu’ should be reconsidered.

关 键 词：金丝楸 遗传多样性 亲缘关系 AFLP 指纹图谱 

分 类 号：S722.7[农业科学—林木遗传育种]