ZFP36和Rasd1的mRNA鉴别生前、死后挫伤的意义  

Forensic application of ZFP36 and Rasd1 mRNAs in identifying antemortem and postmortem skin contusion

作　　者：王寒 高云贵 刘晋岑 赵铁岭 宁波 王起 Wang Han;Gao Yungui;Liu Jincen;Zhao Tieling;Ning Bo;Wang Qi(Jiangxi Jiujiang Judical Expertise Center,Jiujiang 332000,China;Haizhu District Branch of Guangzhou Municipal Public Security Bureau,Guangzhou 510290,China;College of Medicine and Forensics,Xi'an Jiaotong University Health Science Center,Xi'an 710061,China;Foshan Shunde Public Security Bureau Criminal Investigation Brigade,Foshan 528300,China;Department of Forensic Pathology,School of Forensic Medicine,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]江西九江司法鉴定中心,江西九江332000 [2]广州市公安局海珠区分局,广东广州510290 [3]西安交通大学医学部法医学院,陕西西安710061 [4]佛山市顺德区公安局刑侦大队,广东顺德528300 [5]南方医科大学法医学院,广东广州510515

出　　处：《中国法医学杂志》2020年第5期486-489,共4页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81871526)。

摘　　要：目的探索ZFP36和Rasd1在鉴别生前与死后皮肤挫伤上的法医学应用价值。方法使用实时定量聚合酶链式反应(RT-qPCR)的方法,对小鼠和人类皮肤组织中ZFP36和Rasd1的表达情况进行检测。结果在动物实验中,与空白对照和死后挫伤相比,生前挫伤处皮肤ZFP36和Rasd1的mRNA水平明显升高,且二者升高水平在死后72h仍可检出。为进一步验证两者的法医实际应用价值,本实验使用15例法医尸体解剖材料进行了验证。和自身未受损处皮肤相比,死后时间较短(PMI<48h)的10例尸体检材中,有9例ZFP36和Rasd1的mRNA水平在损伤处明显升高;而死亡时间较长(PMI≥60h)的5例尸体材料中,损伤处ZFP36和Rasd1的mRNA水平没有明显变化。结论ZFP36和Rasd1有望作为生前挫伤生活反应的指标应用于法医实践。Objective To explore the forensic application value of ZFP36 and Rasd1 in identifying antemortem and postmortem skin contusion.Methods Through RT-qPCR,this study investigated mRNA levels of ZFP36 and Rasd1 in mouse and human skin wounds.Results In animal experiments,the mRNA levels of skin ZFP36 and Rasd1 increased significantly in antemortem contused mouse skins,compared with blank controls and postmortem contusions.The increased mRNA expressions can still be observed 72 hours after death for ZFP36 and Rasd1 in contused mouse skin,respectively.To further validate the value of both forensic applications,15 postmortem human skin tissues were also examined.In human wounded samples,increased ATF3 and Rasd1 mRNA levels were detected in 9 of 10 cases with short postmortem intervals(PMI<48 h),and there was no significant change in 5 cases with long postmortern intervals(PMI≥60 h).Conclusion These findings suggest that the detection of mRNA levels of ZFP36 and Rasd1 might be used in forensic practice as a potential marker for vital reaction to contusion.

关 键 词：皮肤挫伤 生活反应 ZFP36 Rasd1 RT-QPCR 

分 类 号：DF795.2[医药卫生—法医学]