金刚砂辅助农杆菌进行玉米基因转化研究  

Transgenic Corn Research by Emery-assisted Agrobacterium-Mediated Transformation

作　　者：储坤 聂小熊 冯华鲒 韩桂丽 梁雪莲[1] CHU Kun;NIE Xiao-xiong;FENG Hua-jie;HAN Gui-li;LIANG Xue-lian(College of Life Science,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院生命科学院,广州510225

出　　处：《玉米科学》2020年第5期47-52,共6页Journal of Maize Sciences

基　　金：广东大学生科技创新培育专项基金项目“金刚砂辅助农杆菌进行玉米基因转化创新方法研究”(pdjh2017b0257)。

摘　　要：对超声波处理方式进行改进,在超声波处理时,加入金刚砂以辅助EPSPS基因转导到玉米幼胚中。结果表明,800粒处理种子播种出苗93株,玉米苗经大田glyphosate筛选有27株具有抗性,进行PCR电泳检测,显示23株阳性,初步证明EPSPS基因已经转化到玉米植株中,转化率为29%。金刚砂辅助处理玉米基因转化具有可行性,利于生产实际操作。The ultrasonication-assisted Agrobacterium treatment method was modified with the addition of em⁃ery to increase the treatment intensity to the apical meristem cells of germinating seeds.Ninety three plants from 800 control seeds were sprayed three times with glyphosate solution,and 27 of them survived,their leaves were tak⁃en with a PCR electrophoresis test and 23 shown positive,which suggested that epsps gene was introduced into the corn cultivar,and the transformation rate was 29%.Finally,which has been shown that the method has the advantag⁃es of circumventing the tedious tissue culture procedures and being easy to apply.

关 键 词：玉米 农杆菌 金刚砂 EPSPS基因 

分 类 号：S513.035.3[农业科学—作物学]