鸭坦布苏病毒JJZZ1801株分离鉴定及其E蛋白序列分析  

Isolation and Identification of Duck Tembusu Virus JZ1801 Strain and Sequence Analysis of Viral Envelope Protein

作　　者：梁雄燕[1] 余连喜 孙真真 万亮 陈雪阳 陈晶 方春 顾玉芳[1] 杨玉莹[1] LIANG Xiongyan;YU Lianxi;SUN Zhenzhen;WAN Liang;CHEN Xueyang;CHEN Jing;FANG Chun;GU Yufang;YANG Yuying(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025;Animal Husbandry Bureau of Jiangling County,Jingzhou,Hubei 434101;Jingzhou Huihai Feed Co.,Ltd.,Jingzhou,Hubei 434025)

机构地区：[1]长江大学动物科学学院,湖北荆州434025 [2]江陵县畜牧兽医局,湖北荆州434101 [3]荆州市汇海饲料有限公司,湖北荆州434025

出　　处：《中国家禽》2020年第10期27-32,共6页China Poultry

基　　金：湖北省教育厅科学技术研究计划项目(B2019028);长江大学生创新训练项目(2019118)。

摘　　要：为了解鸭群发病原因,对疑似鸭坦布苏病毒(DTMUV)感染的病鸭进行剖检,采集病变组织进行特异性RT-PCR检测和病原分离鉴定。结果显示:该鸭群为DTMUV感染,从病鸭脑组织分离到一株DTMUV,命名为JZ1801;基因序列分析显示,JZ1801的E基因与泰国分离株DK/TH/CU-1的E基因同源性最高(核苷酸相似度为97.8%),在系统进化树中处于一个新的亚分支,而与我国早期DTMUV分离株同源性相对较低,与之处于不同的亚分支;氨基酸位点差异分析显示,JZ1801的E蛋白氨基酸序列与我国早期分离株FX2010和BYD-1的E蛋白均具有9个差异位点,与疫苗株FX2010-180P的E蛋白存在15个氨基酸位点差异。结果表明近年来我国鸭群中流行的DTMUV E蛋白已经发生了一定程度的变异。In order to determine the cause for the diseased ducks, the suspected duck Tembusu virus(DTMUV)infected ducks were necropsied, and the pathological tissues were sampled for specific RT-PCR and pathogen identification.The results showed that DTMUV infection was confirmed, and one strain of DTMUV was successfully isolated from the brain tissue and named JZ1801. Sequence analysis showed that E gene of JZ1801 exhibited the highest nucleotide homology(97.8%) with that of DK/TH/CU-1 isolated from Thailand and they were classified into a same new sub-branch in phylogenetic tree. But E gene of JZ1801 showed low nucleotide homology with the DTMUV strains isolated in early years in China, which were classified into different sub-branch. And there were 9 amino acids in E protein of JZ1801 different with FX2010 and BYD-1, and 15 amino acids different with the vaccine strain FX2010-180 P. These data indicated that E protein of DTMUV from the ducks circulated in China had obviously mutant in recent years.

关 键 词：鸭坦布苏病毒 分离鉴定 E蛋白 序列分析 

分 类 号：S855.3[农业科学—临床兽医学]