某院耐亚胺培南鲍曼不动杆菌耐药基因及临床特征  

Drug resistance genes and clinical characteristics of imipenem-resistant Acinetobacter baumannii in a hospital

作　　者：姜梅杰[1] 张志军[1] 赵书平[1] 董春忠[2] JIANG Mei-jie;ZHANG Zhi-jun;ZHAO Shu-ping;DONG Chun-zhong(Department of Laboratory Medicine,Taian City Central Hospital,Taian 271000,China;Department of Laboratory Medicine,Peoples’Hospital of Binzhou,Binzhou 256610,China)

机构地区：[1]泰安市中心医院检验科,山东泰安271000 [2]滨州市人民医院检验科,山东滨州256610

出　　处：《中国感染控制杂志》2020年第11期996-1000,共5页Chinese Journal of Infection Control

基　　金：山东省自然科学基金(ZR2016HL44)。

摘　　要：目的分析某院临床分离的耐亚胺培南鲍曼不动杆菌(IRAB)耐药基因携带情况,为医院感染防控和指导临床治疗提供实验室依据。方法采用聚合酶链反应(PCR)方法检测该院临床分离的26株IRAB碳青霉烯类耐药基因(blaIMP、blaKPC、blaNDM-1、blaOXA-23、blaOXA-50、blaOXA-51、blaOXA-58)、氨基糖苷类耐药基因[ant(3″)-Ⅰ、aac(6′)-Ⅰ、armA]和消毒剂耐药基因qacE△1携带情况。结果26株IRAB均携带碳青霉烯酶基因OXA-23和OXA-51,检出率为100%,未检出其他碳青霉烯类耐药基因。氨基糖苷类耐药基因ant(3″)-Ⅰ、aac(6′)-Ⅰ和armA检出率均为96.15%,消毒剂耐药基因qacE△1基因携带率为65.38%。临床分离IRAB菌株92.31%来源于患者的痰液。结论该院临床分离IRAB多数携带相同的碳青霉烯类耐药基因和氨基糖苷类耐药基因,需加强医院感染防控措施。Objective To analyze carrying status of drug-resistant genes of clinically isolated imipenem-resistant Acinetobacter baumannii(IRAB)in a hospital,provide laboratory basis for prevention and control as well as gui-dance for clinical treatment of HAI.Methods Polymerase chain reaction(PCR)was used to detect carrying status of carbapenem-resistant genes(blaIMP,blaKPC,blaNDM-1,blaOXA-23,blaOXA-50,blaOXA-51,and blaOXA-58),aminoglycoside-resistant genes(ant[3″]-Ⅰ,aac[6′]-Ⅰ,armA)and disinfectant-resistant gene qacE△1 of 26 clinically isolated IRAB strains in this hospital.Results 26 strains of IRAB all carried carbapenem-resistant genes OXA-23 and OXA-51,detection rate was 100%,other carbapenem-resistant genes were not found.The detected rates of aminoglycoside-resistant genes ant(3″)-Ⅰ,aac(6′)-Ⅰand armA were all 96.15%,the carrying rate of disinfectant-resistant gene qacE△1 was 65.38%.92.31%of clinically isolated IRAB strains were from sputum of patients.Conclusion The majority of clinically isolated IRAB strains in this hospital carry the same carbapenem-resistant gene and aminoglycoside-resistant gene,it is necessary to strengthen the prevention and control measures of HAI.

关 键 词：鲍曼不动杆菌 耐亚胺培南鲍曼不动杆菌 碳青霉烯类耐药基因 氨基糖苷类耐药基因 消毒剂耐药基因 

分 类 号：R181.32[医药卫生—流行病学]