类风湿关节炎患者Syndecan-4的表达及其与疾病活动度的关系  

Expression of Syndecan-4 in patients with rheumatoid arthritis and its correlation with disease activity

作　　者：叶夏[1] 赵娟[1] 张卓莉[1] YE Xia;ZHAO Juan;ZHANG Zhuo-li(Department of Rheumatology and Clinical Immunology,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院风湿免疫科,北京100034

出　　处：《临床和实验医学杂志》2020年第22期2389-2392,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(编号:81771740,81971524,81901642);北京大学临床研究项目(编号:PUCRP201305)。

摘　　要：目的探讨多配体蛋白聚糖-4(Syndecan-4)在类风湿关节炎(RA)患者血清及关节液中的表达水平,并分析其与疾病活动度的关系。方法回顾性选取2014年10月至2017年10月北京大学第一医院收治病例资料,RA患者的血清(n=20)、关节液(n=25例);骨关节炎(OA)患者的血清(n=20)、关节液(n=25)作为疾病对照组;选取健康志愿者的血清(n=20)作为正常对照组。运用酶联免疫吸附法(ELISA)检测RA组和OA组血清及关节液中Syndecan-4的表达。同时纳入60例不同疾病活动度的RA患者,分析其血清Syndecan-4水平与RA疾病活动之间的相关性。结果RA组血清Syndecan-4水平为637.1(483.6~1069.6)pg/mL,显著高于OA组的345.0(287.9~421.1)pg/mL及正常对照组的195.6(165.0~225.2)pg/mL,差异有统计学意义(均P<0.001)。Syndecan-4在RA和OA患者关节液中表达相当[94.8(23.5~280.3)pg/mL vs.51.0(27.2~163.6)pg/mL],而2组患者关节液中Syndecan-4水平显著低于血清,差异有统计学意义(均P<0.001)。RA患者血清Syndecan-4水平与DAS28-ESR、DAS28-CRP、SDAI、CDAI呈正相关性(分别r=0.487、0.530、0.549、0.558,均P<0.001)。结论RA患者血清Syndecan-4水平高于OA患者。血清Syndecan-4水平与RA疾病活动度相关,Syndecan-4可能在RA的发病机制中发挥重要作用。Objective To investigate the expression of Syndecan-4 in sera and synovial fluid in rheumatoid arthritis(RA)by comparing with osteoarthritis(OA)patients,and to analyze the correlation of Syndecan-4 with RA disease activity.Methods Retrospectively select the data of patients admitted to Peking University First Hospital from October 2014 to October 2017,the serum of RA patients(n=20)and joint fluid(n=25);the serum of patients with osteoarthritis(OA)(n=20)and synovial fluid(n=25)were used as disease control group;serum of healthy volunteers(n=20)was selected as normal control group.The concentrations of syndecan-4 in serum and synovial fluid(SF)were detected by ELISA.At the same time,60 RA patients with different disease activities were included,and the correlation between their serum Syndecan-4 levels and RA disease activities was analyzed.Results The serum Syndedcan-4 concentration in RA patients was 637.1(483.6~1069.6)pg/mL,which was significantly higher than in OA patients 345.0(287.9~421.1)pg/mL and healthy control group 195.6(165.0~225.2)pg/mL,the differences were statistically significant(both P<0.001).The Syndecan-4 concentration in synovial fluid was comparable between RA and OA patients[94.8(23.5~280.3)pg/mL vs.51.0(27.2~163.6)pg/mL].The Syndecan-4 concentration was significantly lower in synovial fluid than in serum,either in RA or in OA patients,the differences were statistically significant(both P<0.001).The level of serum Syndecan-4 in RA patients was positively correlated with DAS28-ESR,DAS28-CRP,SDAI,and CDAI(r=0.487,0.530,0.549,0.558,respectively,all P<0.001).Conclusion The serum Syndecan-4 is higher in RA patients than in OA patients.Serum Syndecan-4 is correlated with disease activity in RA patients.Syndecan-4 may play an important role in the pathogenesis of RA.

关 键 词：类风湿关节炎 骨关节炎 多配体蛋白聚糖-4 疾病活动度 TNF拮抗剂 

分 类 号：R593.22[医药卫生—内科学]