宏转录组测序揭示褐土脲酶基因的表达丰度和细菌来源  

Expression abundances of urease genes and microbe sources of urease in cinnamon soil revealed via metatranscriptomic sequencing

作　　者：胡利伟[1] 轩贝贝 戴华鑫[1] 郭建华[1] 牟文君[1] 刘阳[1] 翟振 闫鼎[2] 蔡宪杰[2] 奚家勤[1] 薛超群[1] 宋纪真[1] HU Liwei;XUAN Beibei;DAI Huaxin;GUO Jianhua;MU Wenjun;LIU Yang;ZHAI Zhen;YAN Ding;CAI Xianjie;XI Jiaqin;XUE Chaoqun;SONG Jizhen(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;Procurement Center,Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]上海烟草集团有限责任公司采购中心,上海市200082

出　　处：《烟草科技》2020年第11期7-14,共8页Tobacco Science & Technology

基　　金：河南省自然科学基金资助项目“基于宏基因组和宏转录组学研究褐土脲酶群落构成与抑制效应分析”(122018AS0020);河南省自然科学基金资助项目“小麦-土壤系统中邻苯二甲酸酯的污染特征及植株响应分子机制研究”(112018AS0030);国家自然科学基金资助-青年科学基金项目“青枯病菌对氟啶胺的抗性机制研究”(122018AZ0330)。

摘　　要：为明确褐土中脲酶编码基因的表达丰度,通过土壤RNA提取和宏转录组测序法,筛选RNA水平上表达较高的脲酶基因,共筛选到122个脲酶结构基因(93个ureC、16个ureB和13个ureA)和脲酶辅助基因115个(29个ureD、12个ureE、36个ureF、29个ureG、5个ureH和4个ureJ)。其中ureB、ureA、ureG、ureH和ureJ所有转录本TPM值都小于2,而ureC、ureD、ureE和ureF丰度最高的转录本TPM值分别为3.17、5.37、2.01和2.71。细菌来源的芽孢杆菌属Bacillus、芽孢八叠球菌属Sporosarcina、节杆菌属Arthrobacter、链霉属Streptomyces和类芽孢杆菌属Paenibacillus等在褐土脲酶活性的发挥中贡献度较大。Previous studies showed that the urease genes responsible for urea degradation in cinnamon soil were from bacteria,fungi and archaea.To clarify the expression abundances of urease genes,soil RNA extraction and metatranscriptomic sequencing were performed to screen urease genes with high expressions in RNA level.A total of 122 structural genes(93 ureC,16 ureB and 13 ureA)and 115 accessory genes(29 ureD,12 ureE,36 ureF,29 ureG,5 ureH and 4 ureJ)were identified.The expression values of ureB,ureA,ureG,ureH and ureJ transcripts were below 2 in TPM(Transcripts per Million),however the highest expression values of ureC,ureD,ureE and ureF were 3.17,5.37,2.01 and 2.71 in TPM respectively.Bacillus,Sporosarcina,Arthrobacter,Streptomyces and Paenibacillus played essential roles in the degradation of urea in cinnamon soil.

关 键 词：褐土 宏转录组测序 脲酶结构基因 脲酶辅助基因 细菌 

分 类 号：TS414[农业科学—烟草工业]