胃癌患者血清IL-37、ANOS1、GKN1水平检测及其临床意义  

Levels of serum IL-37,ANOS1,GKN1 in patients with gastric cancer and its clinical significance

作　　者：朱清 裴小红 黄重发 卜全慧 马小芬 张芳 张海鸣[3] ZHU Qing;PEI Xiaohong;HUANG Chongfa;BU Quanhui;MA Xiaofen;ZHANG Fang;ZHANG Haiming(Department of Gastroenterology,Eastern Theater Air Force Hospital of the PLA,Nanjing,Jiangsu 210000,China;Department of Gastroenterology,the 904th Hospital of the PLA Joint Service Support Force,Suzhou,Jiangsu 215000,China;Department of General Surgery,Affiliated Hospital of Jiangsu University,Zhenjiang,Jiangsu 212000,China)

机构地区：[1]中国人民解放军东部战区空军医院消化内科,江苏南京210000 [2]中国人民解放军联勤保障部队第九〇四医院消化内科,江苏苏州215000 [3]江苏大学附属医院普外科,江苏镇江212000

出　　处：《国际检验医学杂志》2020年第22期2716-2720,共5页International Journal of Laboratory Medicine

基　　金：江苏省自然科学基金面上项目(BK2009208)。

摘　　要：目的探讨血清白细胞介素(IL)-37、ANOS1、胃动蛋白1(GKN1)在胃癌患者中的水平变化及临床意义。方法选择中国人民解放军东部战区空军医院消化内科收治的145例胃癌患者(研究组)和60例体检健康者(对照组)为研究对象,采用酶联免疫吸附试验检测各组受试者血清IL-37、ANOS1、GKN1水平,并进行比较,分析三者与肿瘤最大径、肿瘤组织分化程度、TNM分期、淋巴结转移情况的关系;受试者工作特征(ROC)曲线分析血清IL-37、ANOS1、GKN1对胃癌的诊断价值。结果研究组血清IL-37、ANOS1水平明显高于对照组,血清GKN1水平明显低于对照组,差异均有统计学意义(P<0.05)。肿瘤最大径>4 cm、低分化、TNM分期Ⅲ~Ⅳ期、有淋巴结转移胃癌患者血清IL-37、ANOS1、GKN1水平与肿瘤最大径≤4 cm、中-高分化、TNM分期Ⅰ~Ⅱ期、无淋巴结转移胃癌患者比较,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,IL-37、ANOS1、GKN1联合检测诊断胃癌发生的曲线下面积为0.868,灵敏度和特异度分别为0.895和0.837。多因素Logistic回归分析显示,血清IL-37、ANOS1水平升高,GKN1水平降低是影响胃癌发生的独立危险因素。结论胃癌患者血清IL-37、ANOS1水平升高,血清GKN1水平降低,且与胃癌的发生与进展关系密切,血清IL-37、ANOS1、GKN1联合检测对胃癌诊断有一定临床应用价值。Objective To investigate the changes and clinical significance of serum interleukin-37(IL-37),ANOS1 and motilin 1(GKN1)in patients with gastric cancer.Methods A total of 145 patients with gastric cancer(experimental group)in Department of Gastroenterology,Eastern Theater Air Force Hospital of the Chinese People′s Liberation Army and 60 healthy subjects(control group)were selected as subjects.Enzyme-linked immunosorbent assay was used to detect the serum IL-37,ANOS1 and GKN1 levels of each group,then compared and analyzed the relationships between these indicators and tumor diameter,pathological tissue differentiation,TNM stage and lymph node metastasis.The diagnostic value of serum IL-37,ANOS1 and GKN1 in gastric cancer was analyzed by receiver operator characteristic(ROC)curve.Results The serum IL-37 and ANOS1 levels in experimental group were higher than those in control group,and the serum GKN1 level in experimental group was lower than that in control group,the differences were statistically significant(P<0.05).There were significant differences on serum levels of IL-37,ANOS1,GKN1 between gastric cancer patients with tumor diameter>4 cm,low tumor differentiation,TNM stageⅢ-Ⅳ,lymph node metastasis and patients with tumor diameter≤4 cm,tumor medium-high differentiation,TNM stageⅠ-Ⅱ,no lymph node metastasis(P<0.05).The ROC curve showed that the area under the curve of IL-37,ANOS1,GKN1 combined detection in the diagnosis of gastric cancer was 0.868,the sensitivity and specificity were 0.895 and 0.837 respectively.Multivariate Logistic regression analysis showed that elevated serum IL-37,ANOS1 levels,and decreased GKN1 level were independent risk factors affecting gastric cancer.Conclusion The elevated serum levels of IL-37,ANOS1 and decreased serum level of GKN1 in gastric cancer patients relate to the occurrence and progression of gastric cancer.The combined detection of serum IL-37,ANOS1 and GKN1 has certain clinical application value in the diagnosis of gastric cancer.

关 键 词：胃癌 白细胞介素-37 ANOS1 胃动蛋白1 

分 类 号：R446.6[医药卫生—诊断学]